Bavister B D
Wisconsin Regional Primate Research Center, University of Wisconsin, Madison 53706.
In Vitro Cell Dev Biol. 1988 Aug;24(8):759-63. doi: 10.1007/BF02623645.
Construction details are described for a minichamber device that maintains a localized atmosphere of carbon dioxide in air over the stage of an inverted microscope. This device is easily constructed from Plexiglas and its specifications can be adjusted to fit virtually any inverted microscope. A flow of warm, humidified carbon dioxide in air gas mixture can be directed over a petri dish or unsealed culture flask to maintain the pH of bicarbonate-CO2 buffered media. By this means, prolonged culture of cells directly on the microscope stage is made possible without occurrence of detrimental pH changes. If the microscope is fitted with an environmental control chamber to maintain temperature, cells can be maintained on the microscope stage for days, permitting frequent observation of cell growth and activity. Alternatively, continuous cine or video recordings can be made. For example, using this device, hamster and rhesus monkey embryos have been cultured for 2 to 5 d on an inverted microscope while continuous time-lapse recordings were made of cell division and differentiation and activity of cellular organelles.
本文描述了一种小型培养箱装置的构造细节，该装置可在倒置显微镜载物台上的空气中维持局部二氧化碳气氛。此装置易于用有机玻璃制成，其规格可调整以适配几乎任何倒置显微镜。温暖、湿润的二氧化碳与空气的混合气流可导向培养皿或未密封的培养瓶上方，以维持碳酸氢盐 - 二氧化碳缓冲培养基的pH值。通过这种方式，可以在显微镜载物台上直接对细胞进行长时间培养，而不会出现有害的pH值变化。如果显微镜配备有环境控制箱以维持温度，细胞可以在显微镜载物台上维持数天，便于频繁观察细胞生长和活性。或者，也可以进行连续的电影或视频记录。例如，使用该装置，仓鼠和恒河猴胚胎在倒置显微镜上培养了2至5天，同时对细胞分裂、分化以及细胞器的活动进行了连续的延时记录。
