College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.
Department of Soil Science, Faculty of Agricultural Sciences and Technology, Bahauddin Zakariya University, Multan, Pakistan.
Insect Sci. 2022 Feb;29(1):162-176. doi: 10.1111/1744-7917.12909. Epub 2021 Apr 6.
Chemosensory proteins (CSPs) have great contributions in performing diverse functions in insects. However, physiological appraisal of chemosensory protein genes still remains elusive in insects. We studied expression patterns and binding affinities of MsepCSP14, a chemosensory protein, in Mythimna separata. The distinct functions of MsepCSP14 were validated by employing different molecular techniques. The MsepCSP14 had high resemblance of sequence with chemosensory proteins of other insect family members. The MsepCSP14 expression was higher in antennal tissues of females than other tissues. Fluorescence binding assay validated that binding of nine out of 21 ligands to MsepCSP14 was higher at pH 7.4 than at pH 5.0. Three dimensional modeling (3D) and docking analysis predicted that amino acid residues of MsepCSP14 were involved in binding of compounds, and behavior assay displayed that adults of M. separata considerably responded to four volatiles from compounds demonstrating strong binding ability to MsepCSP14. Results of the present study suggest that MsepCSP14 is likely to mediate chemosensory functions in M. separata.
化学感觉蛋白（CSPs）在昆虫中具有执行多种功能的巨大贡献。然而，昆虫化学感觉蛋白基因的生理评估仍然难以捉摸。我们研究了化学感觉蛋白 MsepCSP14 在粘虫中的表达模式和结合亲和力。通过采用不同的分子技术验证了 MsepCSP14 的不同功能。MsepCSP14 的序列与其他昆虫家族成员的化学感觉蛋白具有高度相似性。MsepCSP14 在雌性触角组织中的表达高于其他组织。荧光结合实验验证了 21 种配体中有 9 种在 pH 值为 7.4 时与 MsepCSP14 的结合高于 pH 值为 5.0 时。三维建模（3D）和对接分析预测 MsepCSP14 的氨基酸残基参与了化合物的结合，行为实验显示 M. separata 的成虫对来自化合物的四种挥发物有明显的反应，对 MsepCSP14 表现出很强的结合能力。本研究结果表明，MsepCSP14 可能在 M. separata 中介导化学感觉功能。
