College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
College of Resources and Environment, Huazhong Agricultural University, Wuhan 430070, China.
Int J Biol Sci. 2018 Nov 1;14(14):1935-1949. doi: 10.7150/ijbs.27315. eCollection 2018.
Chemosensory proteins (CSPs) have been suggested to perform several functions in insects, including chemoreception. To find out whether MsepCSP5 identified from shows potential physiological functions in olfaction, gene expression profiles, ligand-binding experiments, molecular docking, RNA interference, and behavioral test were performed. Results showed that was highly expressed in female antennae. MsepCSP5 showed high binding affinities to a wide range of host-related semiochemicals, and displayed that 26 out of 35 candidate volatiles were highly bound (Ki < 10 µM) at pH 5.0 rather than pH 7.4. The binding sites of MsepCSP5 to candidate volatiles were well predicted by three-dimensional structure modeling and molecular docking experiments. Pursuing further, biological activities of to highly bound compounds elicited strong behavioral responses, such as alcoholic compounds displayed strong attractiveness whereas terpenes showed repellency to . The transcript expression level of MsepCSP5 gene significantly decreased after injecting target dsRNAs, and resulted in non-significant preference responses of to semiochemicals, such as 3-pentanol and 1-octene-3-ol. In conclusion, MsepCSP5 may involve in semiochemical reception of
化感蛋白（CSPs）在昆虫中被认为具有多种功能，包括化学感受。为了探究从 中鉴定出的 MsepCSP5 是否在嗅觉中具有潜在的生理功能，进行了基因表达谱分析、配体结合实验、分子对接、RNA 干扰和行为测试。结果表明， 在雌性触角中高度表达。MsepCSP5 对广泛的与宿主相关的半化学物质表现出高亲和力，并且显示出 35 种候选挥发物中有 26 种在 pH5.0 时（Ki<10µM）而非 pH7.4 时高度结合。MsepCSP5 与候选挥发物的结合位点通过三维结构建模和分子对接实验得到了很好的预测。进一步研究发现， 对高度结合化合物的生物活性引起了强烈的行为反应，例如醇类化合物表现出强烈的吸引力，而萜类化合物对 表现出驱避性。注射靶向 dsRNAs 后，MsepCSP5 基因的转录表达水平显著降低，导致 对半化学物质（如 3-戊醇和 1-辛烯-3-醇）的偏好反应不显著。综上所述，MsepCSP5 可能参与了 对半化学物质的接收。
