ICAR-Directorate of Mushroom Research, Solan, Himachal Pradesh 173213, India.
ICAR-Directorate of Mushroom Research, Solan, Himachal Pradesh-173213, India.
Int J Med Mushrooms. 2021;23(3):91-101. doi: 10.1615/IntJMedMushrooms.2021037856.
Isaria cicadae is an entomopathogenic fungus possessing several therapeutic properties and has a potential role in traditional Chinese medicine. The present study was designed to describe the taxonomic details of a new isolate of I. cicadae collected from the Northern Himalayas of India and to study its vegetative and reproductive growth responses under in vitro conditions. Proximate composition, biochemical profiling, and radical scavenging activities were studied to establish the bioactivity of the isolate. Micromorphological characteristics of conidia and conidiophore formation were studied using scanning electron microscopy. The optimum temperature and pH for mycelial growth was 25°C and 7.0, respectively. Pinhead initiation was observed at day 10 after inoculation, but the fully developed, branched, and coral to club-shaped fruiting bodies could be observed after 30 days of inoculation. Proximate analysis indicated that carbohydrates are the major constituents (50.2%) of the fruit bodies, along with a lower quantity of protein (4.46%), crude fat (6.4%), and crude fiber (1.55%). Vitamin D content of I. cicadae was 3,605.84 IU/g. Radical scavenging activity based on the DPPT (1,1-diphenyl-2-picrylhydrazyl) assay was 21.2%. ABTS [2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid] and potassium ferricyanide reducing activity were quite high, at around 93% and 99.3%, respectively. The findings of this study provide insight into the biochemical constituents of I. cicadae and its cultivation practices for further exploitation of this mushroom at a larger scale.
蝉拟青霉是一种具有多种治疗特性的昆虫病原真菌，在传统中药中有一定的应用潜力。本研究旨在描述从印度喜马拉雅山脉北部采集的一种蝉拟青霉新分离株的分类学细节，并研究其在体外条件下的营养和繁殖生长反应。研究了其近似组成、生化特征和自由基清除活性，以确定其生物活性。使用扫描电子显微镜研究了分生孢子和分生孢子梗形成的微观形态特征。最适的菌丝生长温度和 pH 值分别为 25°C 和 7.0。接种后第 10 天观察到针头状起始，但接种后 30 天可观察到完全发育的、分枝的、珊瑚状到棒状的子实体。近似分析表明，碳水化合物是子实体的主要成分（50.2%），同时还含有较低数量的蛋白质（4.46%）、粗脂肪（6.4%）和粗纤维（1.55%）。蝉拟青霉的维生素 D 含量为 3605.84 IU/g。基于 DPPT（1,1-二苯基-2-苦基肼）测定的自由基清除活性为 21.2%。ABTS[2,2'- 联氮-双(3-乙基苯并噻唑啉-6-磺酸)]和铁氰化钾还原活性相当高，分别约为 93%和 99.3%。本研究的结果为蝉拟青霉的生化成分及其培养实践提供了深入了解，为进一步在更大规模上开发这种蘑菇提供了依据。
