Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Pharmaceutical Science , Jiangnan University , Wuxi 214122 , PR China.
National Engineering Laboratory for Cereal Fermentation Technology , Jiangnan University , Wuxi 214122 , PR China.
J Agric Food Chem. 2019 Aug 7;67(31):8476-8484. doi: 10.1021/acs.jafc.9b02705. Epub 2019 Jul 24.
Cicada flowers, which are edible and medicinal mushrooms, are the fruiting bodies of , a fungus that is parasitic on the larvae of cicada pupae. We hypothesize that host factors might possess stimulatory activity on metabolite synthesis in . Here, we first compared the microbial community structures of different wild cicada flowers across geographical regions, compartments, and growth stages via high-throughput sequencing. TZC-3, an isolate of the most abundant operational taxonomic unit (OTU6782) in all the fungal communities, was isolated from wild cicada flowers. Furthermore, the effects of cicada pupae on metabolite synthesis in TZC-3 were studied in submerged culture. The contents of intercellular polysaccharides, adenosine, 6-(2-hydroxyethyl)-adenosine, free amino acids, and hydrolyzed monosaccharides in the mycelia cultured with cicada pupa powder (4%) were significantly increased as compared with the contents in the control group. This indicates that a cicada pupa can act as an elicitor for metabolite synthesis in .
蝉花，既是食用真菌又是药用真菌，是蝉拟青霉寄生于蝉若虫的子实体。我们推测宿主因子可能对蝉拟青霉代谢产物的合成具有刺激作用。在此，我们首次通过高通量测序，比较了不同地理区域、不同部位和不同生长阶段的野生蝉花的微生物群落结构。TZC-3 是所有真菌群落中最丰富的分类单元（OTU6782）的分离株，从野生蝉花中分离得到。此外，还在液体培养中研究了蝉蛹对 TZC-3 代谢产物合成的影响。与对照组相比，用蝉蛹粉（4%）培养的菌丝体中的胞内多糖、腺苷、6-（2-羟乙基）腺苷、游离氨基酸和水解单糖含量显著增加。这表明蝉蛹可以作为蝉拟青霉代谢产物合成的诱导剂。
