Huang Jiao, Li Minhua, Li Jinhong, Liang Baoyun, Chen Zhaoxia, Yang Jialei, Guo Xiaojing, Huang Siyun, Gu Lian, Su Li
School of Public Health of Guangxi Medical University, 22 Shuangyong Road, Nanning, 530021, Guangxi, China.
Guangxi University of Chinese Medicine, Nanning, Guangxi, China.
Biochem Genet. 2021 Dec;59(6):1359-1380. doi: 10.1007/s10528-021-10055-w. Epub 2021 Apr 7.
Long non-coding RNAs (lncRNAs) have been reported to play an important role in cardiovascular diseases. The present study aimed to investigate the levels of lncRNA H19 in patients with coronary artery disease (CAD) and the genetic association of lncRNA H19 rs217727 and rs4929984 polymorphisms with CAD susceptibility. We detected an upregulated expression of lncRNA H19 in the peripheral blood of CAD patients compared with healthy controls, and the area under the receiver operating characteristic curve of lncRNA H19 for CAD diagnosis was 0.918. In addition, rs4929984 was associated with the susceptibility of Han Chinese females to CAD, as shown in the additive and dominant models, and the significant association remained after adjusting for age and Bonferroni correction. The A allele carriers of rs4929984 were correlated with females' susceptibility to CAD compared with the C allele, and the A-G haplotype of rs4929984-rs217727 was associated with females' susceptibility to CAD. Furthermore, rs217727 and rs4929984 were associated with the levels of clinicopathological parameters of CAD cases. We suggest that lncRNA H19 has a potential to be a diagnostic biomarker for CAD; rs4929984 polymorphism is associated with females' susceptibility to CAD in the Han Chinese population, and lncRNA H19 variants may influence lipid metabolism, inflammation, and coagulation function of CAD patients.
据报道，长链非编码RNA（lncRNAs）在心血管疾病中发挥重要作用。本研究旨在调查冠心病（CAD）患者中lncRNA H19的水平，以及lncRNA H19 rs217727和rs4929984多态性与CAD易感性的遗传关联。与健康对照相比，我们检测到CAD患者外周血中lncRNA H19表达上调，lncRNA H19用于CAD诊断的受试者工作特征曲线下面积为0.918。此外，rs4929984与汉族女性患CAD的易感性相关，如在加性和显性模型中所示，在调整年龄和Bonferroni校正后，这种显著关联仍然存在。与C等位基因相比，rs4929984的A等位基因携带者与女性患CAD的易感性相关，rs49299８4-rs217727的A-G单倍型与女性患CAD的易感性相关。此外，rs217727和rs4929984与CAD病例的临床病理参数水平相关。我们认为lncRNA H19有可能成为CAD的诊断生物标志物；rs4929984多态性与汉族人群中女性患CAD的易感性相关，lncRNA H19变体可能影响CAD患者的脂质代谢、炎症和凝血功能。
