Department of Pharmacology, Xiangya School of Pharmaceutical Science, Central South University, Changsha, China.
Hunan Academy of Traditional Chinese Medicine, Hunan, China.
Pharm Biol. 2020 Dec;58(1):176-183. doi: 10.1080/13880209.2020.1723648.
The pharmacological functions of Wall. ex Lindl. (Orchidaceae) in cardiac hypertrophy remains unclear.
To evaluate whether aqueous extract (DCAE) can attenuate experimental cardiac hypertrophy.
Cardiac hypertrophy in SD rats was induced by subcutaneously injection of isoproterenol (2 mg/kg), once a day for ten days. Rats were gavaged with DCAE (0.13 and 0.78 g/kg) daily for one month. At the end of treatment, measurement of left ventricular systolic pressure (LVSP), heart-to-body weight ratio (HW/BW), left ventricular/tibia length (LV/TL), atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP) levels, haematoxylin-eosin staining, and Masson's trichrome staining were conducted. In cultured H9c2 cells, DCAE (2 mg/mL) and U0126 (10 μM) were added 2 h before the isoproterenol (10 μM) stimulus. Phalloidin staining was used to evaluate cellular hypertrophy. The mRNA expression of ANP and BNP was measured by qRT-PCR. The expression of p-ERK was determined by immunoblotting.
DCAE treatment significantly reduced the following indicators : (1) the LVSP (16%); (2) HW/BW (13%); (3) LV/TL (6%); (4) ANP (39%); (5) BNP (32%). In cultured H9c2 cells, phalloidin staining showed that DCAE relieved cellular hypertrophy (53% reduction). Furthermore, immunoblotting showed that DCAE can significantly inhibit p-ERK protein expression (39% and 27% reduction, respectively).
DCAE prevents cardiac hypertrophy via ERK signalling pathway and has the potential for treatment of cardiac hypertrophy.
石仙桃（兰科）在心脏肥大方面的药理作用尚不清楚。
评估水提物（DCAE）是否能减轻实验性心脏肥大。
通过皮下注射异丙肾上腺素（2mg/kg）每天一次，连续十天诱导 SD 大鼠心脏肥大。大鼠每天灌胃 DCAE（0.13 和 0.78g/kg），连续一个月。治疗结束时，测量左心室收缩压（LVSP）、心脏/体重比（HW/BW）、左心室/胫骨长度比（LV/TL）、心房利钠肽（ANP）、脑利钠肽（BNP）水平、苏木精-伊红染色和 Masson 三色染色。在培养的 H9c2 细胞中，在异丙肾上腺素（10μM）刺激前 2 小时加入 DCAE（2mg/mL）和 U0126（10μM）。鬼笔环肽染色用于评估细胞肥大。通过 qRT-PCR 测量 ANP 和 BNP 的 mRNA 表达。通过免疫印迹测定 p-ERK 的表达。
DCAE 治疗显著降低了以下指标：（1）LVSP（16%）；（2）HW/BW（13%）；（3）LV/TL（6%）；（4）ANP（39%）；（5）BNP（32%）。在培养的 H9c2 细胞中，鬼笔环肽染色显示 DCAE 减轻了细胞肥大（减少 53%）。此外，免疫印迹显示 DCAE 可显著抑制 p-ERK 蛋白表达（分别减少 39%和 27%）。
DCAE 通过 ERK 信号通路预防心脏肥大，具有治疗心脏肥大的潜力。
