[具体药物名称]对小鼠狼疮性肾炎的治疗作用
Therapeutic Effect of on Lupus Nephritis in Mice.
作者信息
Wang Qiang, Sun Peng, Wang Rui, Zhao Xin
机构信息
Chongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing, P.R. China; Department of Biological and Chemical Engineering, Chongqing University of Education, Chongqing, P.R. China; Chongqing Engineering Research Center of Functional Food, Chongqing University of Education, Chongqing, P.R. China; Chongqing Engineering Laboratory for Research and Development of Functional Food, Chongqing University of Education, Chongqing, P.R. China.
出版信息
Pharmacogn Mag. 2017 Jan-Mar;13(49):129-135. doi: 10.4103/0973-1296.197653.
CONTEXT
() widely is a functional drug. The curative effect of on lupus nephritis has been studied .
MATERIALS AND METHOD
The DBA/2 and B6D2F1 mice were used for this experiment. The 50% effective dose (ED) was used to check the effective concentration for this study. Then the SCr, BUN, TC, TG, IL-6, IL-12, TNF-α, and IFN-γ levels were determined by kits. The output of urine protein was determined by means of Coomassie Brilliant Blue, and the auto-antibody dsDNA was determined with titer plate technology and indirect immunofluorescence. The NF-κB, IκB-α, TGF 'β1, Fas, and FasL expressions were measured by RT-PCR and western blot assay. The component analysis of was determined by nuclear magnetic resonance.
RESULTS
Based on the ED result at 329 mg/kg, 200 and 400 mg/kg doses were chosen for this study. SCr, BUN, TC and TG levels of 400 mg/kg mice were lower than control mice, TP and ALB levels were higher than control mice. The control and 400 mg/kg treated mice tested positive for dsDNA at the end of sixth and tenth week after the experiment began. The glomerular number of 400 mg/kg treated mice was more than control group. Treatment with 400 mg/kg reduced IL-6, IL-12, TNF-α and IFN-γcytokine levels as compared to control mice. decreased NF-κb, TGF 'β1, Fas, FasL and increased IκB-α expressions in kidney tissue. There were 11 compounds in dry , these compounds might make the curative effects of lupus nephritis.
CONCLUSION
showed a potential curative effect on lupus nephritis. It could be used as a health medicine on lupus nephritis.
SUMMARY
reduced the SCr, BUN, TC, TG serum levels and raised the TP, ALB levels compared to control group.The glomerular number of treated mice was more than control group. treated mice showed lower IL-6, IL-12, TNF-α and IFN-γ cytokine levels than control mice. decreased NF-κb, TGF-β1, Fas, FasL and increased IκB-α expressions in kidney tissue. LN: Lupus nephritis, SLE: systemic lupus erythematosus, : ; IL-6: interleukin-6, IL-12: interleukin-12, TNF-α: tumor necrosis factor alpha, IFN-γ: Interferon-gamma, SCr: serum creatinine, BUN: blood urea nitrogen, TC: total cholesterol, TG: triglyceride, TP: total protein, ALB: albumin.
背景
（）广泛是一种功能性药物。已对其治疗狼疮性肾炎的疗效进行了研究。
材料与方法
本实验使用DBA/2和B6D2F1小鼠。采用50%有效剂量（ED）来确定本研究的有效浓度。然后通过试剂盒测定血清肌酐（SCr）、血尿素氮（BUN）、总胆固醇（TC）、甘油三酯（TG）、白细胞介素-6（IL-6）、白细胞介素-12（IL-12）、肿瘤坏死因子-α（TNF-α）和干扰素-γ（IFN-γ）水平。采用考马斯亮蓝法测定尿蛋白输出量，并用滴定板技术和间接免疫荧光法测定自身抗体双链DNA（dsDNA）。通过逆转录聚合酶链反应（RT-PCR）和蛋白质免疫印迹法测定核因子-κB（NF-κB）、核因子κB抑制蛋白α（IκB-α）、转化生长因子-β1（TGF-β1）、Fas和Fas配体（FasL）的表达。通过核磁共振确定（）的成分分析。
结果
基于329mg/kg的ED结果，本研究选择了200mg/kg和400mg/kg的剂量。400mg/kg组小鼠的SCr、BUN、TC和TG水平低于对照组小鼠，总蛋白（TP）和白蛋白（ALB）水平高于对照组小鼠。在实验开始后的第六周和第十周结束时，对照组和400mg/kg治疗组小鼠的dsDNA检测均呈阳性。400mg/kg治疗组小鼠的肾小球数量多于对照组。与对照组小鼠相比，400mg/kg（）治疗可降低IL-6、IL-12、TNF-α和IFN-γ细胞因子水平。（）可降低肾脏组织中NF-κB、TGF-β1、Fas、FasL的表达，并增加IκB-α的表达。干燥的（）中有11种化合物，这些化合物可能产生狼疮性肾炎的治疗效果。
结论
（）对狼疮性肾炎显示出潜在的治疗效果。它可作为治疗狼疮性肾炎的保健药物。
总结
与对照组相比，（）降低了SCr, BUN, TC, TG血清水平，并提高了TP, ALB水平。（）治疗组小鼠的肾小球数量多于对照组。（）治疗组小鼠的IL-6, IL-12, TNF-α和IFN-γ细胞因子水平低于对照组小鼠。（）可降低肾脏组织中NF-κB、TGF-β1、Fas、FasL的表达，并增加IκB-α的表达。LN：狼疮性肾炎，SLE：系统性红斑狼疮，（）：；IL-6：白细胞介素-6，IL-12：白细胞介素-12，TNF-α：肿瘤坏死因子-α，IFN-γ：干扰素-γ，SCr：血清肌酐，BUN：血尿素氮，TC：总胆固醇，TG：甘油三酯，TP：总蛋白，ALB：白蛋白
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