Jibril Fatima Ibrahim, Mohd Hilmi Abu Bakar, Aliyu Sani
School of Biomedicine, Faculty of Health Sciences, Universiti Sultan Zainal Abidin, Terengganu, Malaysia.
Department of Microbiology, Umaru Musa Yar'adua University Katsina, Katsina, Nigeria.
J Pharm Bioallied Sci. 2020 Nov;12(Suppl 2):S831-S835. doi: 10.4103/jpbs.JPBS_280_19. Epub 2020 Nov 5.
Stingless bee is an insect that belongs to the family Apidae. Its name is based on its disability of stinging. It has a high product of honey and propolis by which are commonly referred to as stingless bee honey and stingless bee propolis. honey is one of the crucial natural sources and has the potential to kill infectious microorganisms. Previous studies have proved that the antibacterial activity of natural honey was an effect of hydrogen peroxide, a substance contained in the honey. However, these claims were contradicting with too many studies.
Therefore, this study aimed to identify the antibacterial activity of Malaysian honey which contained non-hydrogen peroxide against , an opportunistic microbial.
honey was used as an antibacterial agent for the treatment of in agar well diffusion assay. An amplex red hydrogen peroxide kit was used to identify the hydrogen peroxide in the honey sample. Meanwhile, non-hydrogen peroxide activity was performed by using honey-catalase treated.
For the first time, we found that hydrogen peroxide was absent in all honey samples. honey has higher antibacterial activity (13.30 ± 0.56mm) compared to honey (9.03 ± 0.22mm) in agar well diffusion assay.
Non-hydrogen peroxide in honey is a bioactive compound and beneficial to kill the microbial infection.
Antibacterial activity of Malaysian honey is directly contributed by non-hydrogen peroxide.
无刺蜂是一种属于蜜蜂科的昆虫。它的名字源于其无蜇刺的能力。它能高产蜂蜜和蜂胶，通常被称为无刺蜂蜂蜜和无刺蜂蜂胶。蜂蜜是重要的天然来源之一，具有杀死传染性微生物的潜力。先前的研究证明，天然蜂蜜的抗菌活性是蜂蜜中所含过氧化氢的作用。然而，这些说法与太多研究相矛盾。
因此，本研究旨在鉴定马来西亚不含过氧化氢的蜂蜜对一种机会性微生物的抗菌活性。
在琼脂孔扩散试验中，将蜂蜜用作抗菌剂来处理[具体微生物名称未给出]。使用安普利克斯红过氧化氢试剂盒来鉴定蜂蜜样品中的过氧化氢。同时，通过用蜂蜜 - 过氧化氢酶处理来检测不含过氧化氢的活性。
首次发现所有[具体蜂蜜品种未给出]蜂蜜样品中均不存在过氧化氢。在琼脂孔扩散试验中，[具体蜂蜜品种未给出]蜂蜜（抑菌圈直径13.30±0.56毫米）相比[具体蜂蜜品种未给出]蜂蜜（抑菌圈直径9.03±0.22毫米）具有更高的抗菌活性。
[具体蜂蜜品种未给出]蜂蜜中的非过氧化氢是一种生物活性化合物，有利于杀死微生物感染。
马来西亚[具体蜂蜜品种未给出]蜂蜜的抗菌活性直接由非过氧化氢所致。
