Li Shuang, Xu Chaomin, Du Guilin, Wang Guangjun, Tu Xiongbing, Zhang Zehua
State Key Laboratory of Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
Scientific Observation and Experimental Station of Pests in Xilin Gol Rangeland, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Xilinhot, China.
Front Physiol. 2021 Mar 22;12:642893. doi: 10.3389/fphys.2021.642893. eCollection 2021.
In order to explore the synergistic control effect of crude extracts of and on , we used different doses of and crude extracts of singly and in combination, to determine their toxicities to fourth instar . The results showed that the combination of 10% crude extract of with 10 and 10 spores/g concentrations and the combination of 20% crude extract of with 10 and 10 spores/g concentrations had significant effects on the mortality, body weight gain, body length gain, growth rate, and overall performance of than those of the crude extract of and alone. Among them, the 20% crude extract mixed with 10 spores/g and 10% crude extract combined with 10 spores/g , had the best control efficacy. In order to clarify the biochemical mechanism underlying the immune responses of to the pesticide treatments, we monitored the activities of four enzymes: superoxidase dismutase (SOD), peroxidase (POD), catalase (CAT), and polyphenol oxidase (PPO). The results showed that the activities of three enzymes (SOD, CAT, and PPO) were significantly increased from the treatment with the combination of mixed with crude extract of . Interestingly, compared to the crude extract, the combination treatment did not significantly induce the expression of POD enzyme activity, which may be a biochemical factor for increasing the control effect of the combination treatment. Our results showed that the combination treatment had synergistic and antagonistic effects on host mortality, growth, development, and enzyme activities in .
为了探究[具体两种提取物名称]粗提物对[具体对象名称]的协同控制效果，我们单独及联合使用不同剂量的[具体提取物名称]粗提物和[具体提取物名称]，以测定它们对四龄[具体对象名称]的毒性。结果表明，10%的[具体提取物名称]粗提物与10和10孢子/克[具体物质名称]浓度组合，以及20%的[具体提取物名称]粗提物与10和10孢子/克[具体物质名称]浓度组合，对[具体对象名称]的死亡率、体重增加、体长增加、生长速率和整体性能的影响，比单独使用[具体提取物名称]粗提物和[具体提取物名称]时更为显著。其中，20%的[具体提取物名称]粗提物与10孢子/克[具体物质名称]混合以及10%的[具体提取物名称]粗提物与10孢子/克[具体物质名称]组合，具有最佳的防治效果。为了阐明[具体对象名称]对农药处理免疫反应的生化机制，我们监测了四种酶的活性：超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）和多酚氧化酶（PPO）。结果表明，三种酶（SOD、CAT和PPO）的活性在[具体提取物名称]与[具体提取物名称]粗提物混合处理后显著增加。有趣的是，与粗提物相比，联合处理并未显著诱导POD酶活性的表达，这可能是联合处理防治效果增强的一个生化因素。我们的结果表明，联合处理对[具体对象名称]宿主的死亡率、生长、发育和酶活性具有协同和拮抗作用。
