Cell Biology, Deutsches Rheuma-Forschungszentrum Berlin, Institute of the Leibniz Association, Berlin, Germany.
Central Lab for Microscopy, Deutsches Rheuma-Forschungszentrum Berlin, Institute of the Leibniz Association, Berlin, Germany.
Front Immunol. 2021 Mar 22;12:624013. doi: 10.3389/fimmu.2021.624013. eCollection 2021.
Studies in animal models have shown that skin tissue-resident memory T (T) cells provide enhanced and immediate effector function at the site of infection. However, analyses of skin T cells in humans have been hindered by the lack of an optimized isolation protocol. Here, we present a combinatorial strategy-the 6-h collagenase IV digestion and gentle tissue dissociation - for rapid and efficient isolation of skin T cells with skin tissue-specific immune features. In comparison with paired blood circulating memory T cells, these isolated skin T cells express typical T cell markers and display higher polyfunctional properties. Moreover, these isolated cells can also be assessed for longer periods of time in cultures. Thus, the optimized isolation protocol provides a valuable tool for further understanding of human skin T cells, especially for direct comparison with peripheral blood T cells at the same sample collection time.
动物模型研究表明，皮肤组织驻留记忆 T（T）细胞在感染部位提供增强和即时的效应功能。然而，对人类皮肤 T 细胞的分析受到缺乏优化分离方案的阻碍。在这里，我们提出了一种组合策略-6 小时胶原酶 IV 消化和温和的组织解离-用于快速有效地分离具有皮肤组织特异性免疫特征的皮肤 T 细胞。与配对的循环血液记忆 T 细胞相比，这些分离的皮肤 T 细胞表达典型的 T 细胞标记物，并表现出更高的多功能特性。此外，这些分离的细胞还可以在培养物中更长时间地进行评估。因此，优化的分离方案为进一步了解人类皮肤 T 细胞提供了有价值的工具，特别是在同一样本采集时间与外周血 T 细胞进行直接比较时。
