Yahya Ahmed Mohammed, Abdalbagi Ali Hashim, Mohammed Taher Gorish Babbiker, Omer Ali Sara, Saif Aldein Abdalrhim Eman, Hamza Mergani Mawada, Abass Abd Elgadir Asmaa, Khalid Mohammed Somaya, Omer Ahmed Salma, Alsaeid Musa Naglaa, Saeed Ahmed Alaa, Mohammed Abdalla Wafaa, Fadlallah Hamedelnil Yousif, Ibrahim Hashim Ahmed, Altayb Hisham N
Department of Microbiology, Faculty of Medical Laboratory Science, Sudan University of Science and Technology, Khartoum, Sudan.
Department of Microbiology, Faculty of Medical Laboratory Science, Omdurman Islamic University, Omdurman, Sudan.
Int J Microbiol. 2021 Mar 19;2021:5520573. doi: 10.1155/2021/5520573. eCollection 2021.
Staphylococcal food poisoning is an intoxication that results from the consumption of improperly prepared or stored foods containing sufficient amounts of one or more preformed . enterotoxins. Nowadays, many researchers worldwide noted an emergence of resistant strains such as particularly for the antibiotic methicillin. Therefore, this study was aimed to determine the existence of and its enterotoxins, mecA genes, in selected food samples. A total of 400 selected food samples were collected from different areas in Khartoum State. The selected foods included cheese, meat products, fish, and raw milk. One hundred samples from each type of food were cultivated, and the resultant growth yielded 137 (34.25%) . , 126 (31.5%) bacteria other than . , and 137 (34.25%) yielded no growth. Eighty-four of the 137 . isolates were randomly selected and tested for the presence of mecA and enterotoxin genes. The oxacillin sensitivity test showed that 15 (11%) of 137 . isolates were oxacillin resistant. The PCR assay showed that the mecA gene was detected in 15 of 84 (17%) . isolates. Simultaneously, only 2 (2.385%) out of 84 . isolates showed an enterotoxin B gene product. There was a relatively moderate prevalence of methicillin-resistant with very low frequency of enterotoxin B gene in different kinds of selected food samples collected from Khartoum State. These findings elucidate the increased risk on public in Khartoum being affected by Staphylococcal food poisoning upon consumption of dairy or meat products prepared in unhygienic conditions that could lead to intoxication by enterotoxins.
葡萄球菌食物中毒是一种中毒现象，它是由于食用了制备不当或储存不当、含有足够量一种或多种预先形成的肠毒素的食物所致。如今，世界各地的许多研究人员都注意到了耐药菌株的出现，尤其是对甲氧西林耐药的菌株。因此，本研究旨在确定从选定的食品样本中是否存在金黄色葡萄球菌及其肠毒素、mecA基因。总共从喀土穆州的不同地区收集了400份选定的食品样本。选定的食品包括奶酪、肉制品、鱼类和生牛奶。每种食品选取100个样本进行培养，培养结果显示，有137个（34.25%）样本培养出金黄色葡萄球菌，126个（31.5%）样本培养出除金黄色葡萄球菌以外的细菌，137个（34.25%）样本未生长出细菌。从137个金黄色葡萄球菌分离株中随机选取84个，检测其mecA和肠毒素基因的存在情况。苯唑西林敏感性试验表明，137个金黄色葡萄球菌分离株中有15个（11%）对苯唑西林耐药。聚合酶链反应检测显示，84个金黄色葡萄球菌分离株中有15个（17%）检测到mecA基因。同时，84个金黄色葡萄球菌分离株中只有2个（2.385%）显示出肠毒素B基因产物。在从喀土穆州收集的不同种类的选定食品样本中，耐甲氧西林金黄色葡萄球菌的患病率相对中等，而肠毒素B基因的频率非常低。这些发现表明，喀土穆的公众在食用卫生条件差的乳制品或肉制品时，受葡萄球菌食物中毒影响的风险增加，这可能导致因金黄色葡萄球菌肠毒素而中毒。
