从埃及食物中毒爆发中分离的耐甲氧西林金黄色葡萄球菌中肠毒素基因的分子特征。

Molecular characterization of enterotoxin genes in methicillin-resistant S. aureus isolated from food poisoning outbreaks in Egypt.

机构信息

Department of Microbiology and Immunology, Faculty of Pharmacy, Delta University for Science and Technology, Gamasa, 11152, Egypt.

Microbiology and Immunology Department, Faculty of Pharmacy, Mansoura University, Mansoura, 35516, Egypt.

出版信息

J Health Popul Nutr. 2023 Aug 28;42(1):86. doi: 10.1186/s41043-023-00416-z.

DOI:10.1186/s41043-023-00416-z

PMID:37641155

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10463939/

Abstract

BACKGROUND

Staphylococcus aureus (S. aureus), especially methicillin-resistant S. aureus (MRSA), is a known disease-causing bacteria with many associated health hazards. Staphylococcal food poisoning can result from staphylococcal enterotoxins (SEs).

METHODS

In this study, 50 S. aureus isolates were isolated from the gastrointestinal tract (GIT) clinical samples of patients with food poisoning in clinical laboratories at Mansoura University Hospital, Egypt. For determination their antibiogram, these isolates were tested for antimicrobial sensitivity against 12 antimicrobial agents using the agar disk diffusion test. After DNA extraction from the isolates, conventional polymerase chain reaction (PCR) was used to detect mecA and SEs genes.

RESULTS

As a result, all isolates were ampicillin and cefoxitin-resistant, while 86% (43 of 50) of the tested isolates exhibited multidrug resistance (MDR). In contrast, the highest sensitivity was confirmed against vancomycin, linezolid and quinolones, namely ciprofloxacin and norfloxacin. Although 100% of the isolates were mecA positive, staphylococcal enterotoxin genes set-A, set-B, set-C, set-G, set-M, and set-O genes were detected in 56%, 20%, 8%, 32%, 16%, and 24%, of the tested isolates, respectively. Finally, isolates encompassing SEs genes were used to validate a microarray chip, indicating its potential for a better methodological approach for detecting and identifying SEs in human samples.

CONCLUSION

The genotypic findings of this study may help explain the enterotoxigenic patterns in S. aureus among Egyptian patients with food poisoning.

摘要

背景

金黄色葡萄球菌（S. aureus），特别是耐甲氧西林金黄色葡萄球菌（MRSA），是一种已知的致病细菌，存在许多相关的健康风险。葡萄球菌食物中毒可由葡萄球菌肠毒素（SEs）引起。

方法

本研究从埃及曼苏拉大学医院临床实验室的食物中毒患者胃肠道（GIT）临床样本中分离出 50 株金黄色葡萄球菌。为了确定其抗生素敏感性，使用琼脂平板扩散试验对这些分离株进行了 12 种抗菌药物的药敏试验。从分离株中提取 DNA 后，使用常规聚合酶链反应（PCR）检测 mecA 和 SEs 基因。

结果

所有分离株均对氨苄西林和头孢西丁耐药，而 86%（50 株中的 43 株）的测试分离株表现出多药耐药（MDR）。相比之下，对万古霉素、利奈唑胺和喹诺酮类药物（即环丙沙星和诺氟沙星）的敏感性最高。尽管 100%的分离株 mecA 阳性，但在测试的分离株中分别检测到葡萄球菌肠毒素基因集-A、-B、-C、-G、-M 和-O 的 56%、20%、8%、32%、16%和 24%。最后，包含 SEs 基因的分离株用于验证微阵列芯片，表明其在检测和鉴定人类样本中 SEs 方面具有更好的方法学方法的潜力。