对干旱胁迫下番茄转录组进行从头组装，有助于深入了解其耐受机制。 （注：原文中“L.”推测有误，根据语境可能是“Lycopersicon”即番茄属，这里按番茄属翻译，若原文无误请根据实际正确物种名修改）
De novo assembly of L. transcriptome in response to drought stress provides insight into the tolerance mechanisms.
作者信息
Sun Xinzhu, Hu Songmiao, Wang Xin, Liu He, Zhou Yun Wei, Guan Qingjie
机构信息
Key Laboratory of Saline-Alkali Vegetation Ecology Restoration, Ministry of Education, Northeast Forest University, Harbin, China.
Garden College, Northeast Forest University, Harbin, China.
出版信息
PeerJ. 2021 Mar 22;9:e11044. doi: 10.7717/peerj.11044. eCollection 2021.
BACKGROUND
L. is a deciduous shrub that is native to North America and has been introduced to China as an ornamental plant. In order to clarify the drought resistance characteristics of L. and excavate the related genes involved in drought resistance regulation pathway, the mechanism of drought resistance stress of L. was revealed by the changes of transcriptome of L. under drought stress.Through the changes of the transcriptome of L. under drought stress, the mechanism of anti-stress of L. could be revealed.
METHODS
Different concentrations of polyethylene glycol-6000 (PEG-6000) was used to simulate drought stress, and transcriptomic analysis was used to reveal the changes of gene expression patterns in L. seedlings.
RESULTS
Results showed that L. seedlings were seriously affected by PEG-6000. As for the differently expressed genes (DEGs), most of them were up-regulated. The additional Go and KEGG analysis results showed that DEGs were functionally enriched in cell wall, signal transduction and hormonal regulation related pathways. DEGs like AfSOD, AfHSP, AfTGA, AfbZIP and AfGRX play roles in response to drought stress.
CONCLUSION
In conclusion, L. seedlings were sensitive to drought, which was different from L. tree, and the genes functions in drought stress responses via ABA-independent pathways. The up-regulation of Salicylic acid signal related DEGs (AfTGA and AfPR-1) indicated that Salicylic acid play a key role in response to drought stress in L.
背景
L. 是一种原产于北美的落叶灌木，已作为观赏植物引入中国。为了阐明L. 的抗旱特性并挖掘参与抗旱调控途径的相关基因，通过干旱胁迫下L. 的转录组变化揭示了L. 的抗旱胁迫机制。通过干旱胁迫下L. 的转录组变化，可以揭示L. 的抗逆机制。
方法
使用不同浓度的聚乙二醇 - 6000（PEG - 6000）模拟干旱胁迫，并利用转录组分析揭示L. 幼苗中基因表达模式的变化。
结果
结果表明，L. 幼苗受到PEG - 6000的严重影响。至于差异表达基因（DEGs），大多数呈上调状态。额外的基因本体（Go）和京都基因与基因组百科全书（KEGG）分析结果表明，DEGs在细胞壁、信号转导和激素调节相关途径中功能富集。诸如AfSOD、AfHSP、AfTGA、AfbZIP和AfGRX等DEGs在响应干旱胁迫中发挥作用。
结论
总之，L. 幼苗对干旱敏感，这与L. 树不同，并且这些基因通过不依赖脱落酸（ABA）的途径在干旱胁迫响应中发挥作用。水杨酸信号相关DEGs（AfTGA和AfPR - 1）的上调表明水杨酸在L. 对干旱胁迫的响应中起关键作用。
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