Drought resistance of tobacco overexpressing the gene of Linn.

Plants are often adversely affected by abiotic stresses such as drought, low temperature, and salinity during growth, and plant NAC-like transcription factors are involved in regulating growth and developmental processes in response to stresses such as drought and salinity. In this study, to investigate the function of , a co-expression network of genes was constructed using gene expression data from the Chinese legume deciduous shrub, Linn. A 576 bp NAC transcription factor ( gene, MN180266) encoding 191 amino acids was isolated from seedlings by RT-PCR. qRT-PCR showed that the gene was expressed in the roots, stems, leaves, and flowers of However, drought stress significantly increased root expression, and the AfNAC1 protein was localized in the nucleus by green fluorescence detection. This study analyzed the drought resistance of overexpressing tobacco in depth. Under natural drought stress, the chlorophyll and antioxidant enzyme activities of overexpressing plants were significantly higher than those of wild-type (WT) plants, but the MDA content was lower than that of WT; after rehydration the Fv/Fm values of -overexpressing tobacco recovered faster than those of wild-type tobacco and rapidly reached the control levels; may be involved in the regulation of the photosystem and indirectly in the regulation of the plant in response to drought stress.