Evaluation of Bacterial Load and Antibiotic Resistance Pattern of from Ready-to-Eat Raw Beef in Bahir Dar City, Ethiopia.

BACKGROUND

S is one of the most important causes of food-borne intoxication and the most frequent antibiotic-resistant pathogen in the world. Regular evaluation of the current safety status of food is a proactive measure to minimize the possible danger of food-borne pathogens. Therefore, this study was conducted to assess the bacterial load and antibiotic resistance profile of from ready-to-eat raw beef in Bahir Dar city, Ethiopia. . This cross-sectional study was conducted from October 2018 to April 2019 by collecting a total of 101 raw beef samples from butcher shops using a simple random sampling method. Isolation and microbial load determination of use were performed by conventional culture method and an antibiotic susceptibility test was conducted by using Kirby Bauer disk diffusion method on the Mueller-Hinton agar. The data were analyzed by using STATA software version 12.0.

RESULT

Out of 101 raw beef samples, 55 (54.45%) were positive for with a mean bacterial count of 3.40 ± 0.63 (log cfu/g). About 13% of butcher shops had unacceptable and potentially dangerous (above 10 cfu/g) bacterial load. High drug resistance was observed on penicillin (92.73%) followed by cefoxitin (74.5%), tetracycline (63.63%), and clindamycin (50.9%). On the other hand, there was the highest susceptibility for ciprofloxacin (100%) followed by gentamycin (90.91%) and erythromycin (87.27%). Multidrug resistance was also found in 54 (98%) of the isolates.

CONCLUSION

In this study highly drug-resistant was incriminated as the main meat contaminant in butcheries of Bahir Dar city. Therefore, appropriate antimicrobial use and staphylococcal control methods should be employed to prevent intoxications in foods.