Tsehayneh Bizuneh, Yayeh Taddesse, Agmas Birhan
Department of Veterinary Science, College of Agriculture and Environmental Sciences, Bahir Dar University, Bahir Dar, Ethiopia.
Int J Microbiol. 2021 Mar 25;2021:5560596. doi: 10.1155/2021/5560596. eCollection 2021.
S is one of the most important causes of food-borne intoxication and the most frequent antibiotic-resistant pathogen in the world. Regular evaluation of the current safety status of food is a proactive measure to minimize the possible danger of food-borne pathogens. Therefore, this study was conducted to assess the bacterial load and antibiotic resistance profile of from ready-to-eat raw beef in Bahir Dar city, Ethiopia. . This cross-sectional study was conducted from October 2018 to April 2019 by collecting a total of 101 raw beef samples from butcher shops using a simple random sampling method. Isolation and microbial load determination of use were performed by conventional culture method and an antibiotic susceptibility test was conducted by using Kirby Bauer disk diffusion method on the Mueller-Hinton agar. The data were analyzed by using STATA software version 12.0.
Out of 101 raw beef samples, 55 (54.45%) were positive for with a mean bacterial count of 3.40 ± 0.63 (log cfu/g). About 13% of butcher shops had unacceptable and potentially dangerous (above 10 cfu/g) bacterial load. High drug resistance was observed on penicillin (92.73%) followed by cefoxitin (74.5%), tetracycline (63.63%), and clindamycin (50.9%). On the other hand, there was the highest susceptibility for ciprofloxacin (100%) followed by gentamycin (90.91%) and erythromycin (87.27%). Multidrug resistance was also found in 54 (98%) of the isolates.
In this study highly drug-resistant was incriminated as the main meat contaminant in butcheries of Bahir Dar city. Therefore, appropriate antimicrobial use and staphylococcal control methods should be employed to prevent intoxications in foods.
金黄色葡萄球菌是食源性中毒的最重要原因之一，也是世界上最常见的耐药病原体。定期评估食品当前的安全状况是将食源性病原体的潜在危险降至最低的积极措施。因此，本研究旨在评估埃塞俄比亚巴赫达尔市即食生牛肉中金黄色葡萄球菌的细菌载量和抗生素耐药性特征。本横断面研究于2018年10月至2019年4月进行，采用简单随机抽样方法从肉店共采集101份生牛肉样本。金黄色葡萄球菌的分离和微生物载量测定采用传统培养方法，抗生素敏感性试验采用Kirby Bauer纸片扩散法在Mueller-Hinton琼脂上进行。数据使用STATA软件12.0版进行分析。
在101份生牛肉样本中，55份（54.45%）金黄色葡萄球菌检测呈阳性，平均细菌计数为3.40±0.63（log cfu/g）。约13%的肉店细菌载量不可接受且有潜在危险（高于10 cfu/g）。观察到对青霉素的耐药性最高（92.73%），其次是头孢西丁（74.5%）、四环素（63.63%）和克林霉素（50.9%）。另一方面，对环丙沙星的敏感性最高（100%），其次是庆大霉素（90.91%）和红霉素（87.27%）。在54份（98%）分离株中也发现了多重耐药性。
在本研究中，高耐药性金黄色葡萄球菌被认为是巴赫达尔市肉店中主要的肉类污染物。因此，应采用适当的抗菌药物使用和葡萄球菌控制方法来预防食品中的金黄色葡萄球菌中毒。
