健康志愿者和艾滋病患者血清中可溶性 HLA Ⅰ类抗原的定量分析。

Quantification of soluble serum HLA class I antigens in healthy volunteers and AIDS patients.

作者信息

Ferreira A, Villar M L, Alvarez Cermeño J C, Revilla Y, García Rodriguez M C, Fontán G, González-Porqué P

机构信息

Unidad de Immunología, Ciudad Sanitaria La Paz, Madrid, Spain.

出版信息

Clin Chim Acta. 1988 May 31;174(2):207-11. doi: 10.1016/0009-8981(88)90387-7.

DOI:10.1016/0009-8981(88)90387-7

PMID:3383444

Abstract

A solid-phase enzyme immunoassay (ELISA) has been used to quantify human soluble Class I histocompatibility antigens in serum samples from voluntary blood donors and AIDS patients. Statistical analysis of the results showed significantly raised levels (p less than 0.01) of free HLA Class I in sera from AIDS patients (2.95 +/- 1.80 micrograms/ml) when compared with the blood donors (1.06 +/- 0.6 micrograms/ml). The assay is specific, reproducible and easy to perform. Potential uses of this determination are discussed.

摘要

一种固相酶免疫测定法（ELISA）已用于定量检测自愿献血者和艾滋病患者血清样本中的人类可溶性I类组织相容性抗原。结果的统计分析显示，与献血者（1.06±0.6微克/毫升）相比，艾滋病患者血清中游离HLA I类抗原水平显著升高（p<0.01）（2.95±1.80微克/毫升）。该测定法具有特异性、可重复性且易于操作。文中讨论了这种检测方法的潜在用途。