Toder V, Madanes A, Gleicher N
Division of Reproductive Immunology, Mount Sinai Hospital Medical Center, Chicago, IL 60608.
Contraception. 1988 Apr;37(4):391-403. doi: 10.1016/0010-7824(88)90116-3.
Embryo implantation has been demonstrated to depend on specific lymphocyte populations within the uterine cavity. Intrauterine devices (IUD) exert their contraceptive action by prevention of embryo implantation via presently still unknown mechanisms. Therefore, mononuclear cell populations from mice uteri which either contained silastic or copper (Cu) IUD fragments or were sham-operated were evaluated, utilizing monoclonal antibodies against specific cell markers. Uterine horns, bearing IUD fragments, were significantly heavier than sham-operated horns. In Cu-IUD animals this effect extended even into the non-treated contralateral horn. The total number of lymphoid cells in IUD-bearing horns was significantly higher than in sham-operated horns. This observation was also made in non-treated contralateral Cu-IUD horns but not in contralateral horns of silastic-IUD-treated animals. Significant differences in percentages as well as absolute number of various lymphoid cell populations were noted between IUD-treated and sham-operated animals. Again, the effect was more pronounced in Cu-IUD-treated animals and extended in those animals into the contralateral horn. IUD-containing horns also demonstrated a significantly increased number of mast cells, with Cu-IUDs again resulting in a significantly more pronounced effect in both treated and contralateral horns. Sham-operated mice achieved a 67% pregnancy rate in both uterine horns. In contrast, IUD-treated animals demonstrated a significantly reduced pregnancy rate with silastic IUD fragments (15% and 30% for treated and contralateral horn, respectively) and a 0% pregnancy rate for Cu-IUD-treated animals (in either horn).
胚胎着床已被证明依赖于子宫腔内特定的淋巴细胞群体。宫内节育器（IUD）通过目前仍不清楚的机制阻止胚胎着床来发挥其避孕作用。因此，利用针对特定细胞标志物的单克隆抗体，对来自含有硅橡胶或铜（Cu）宫内节育器碎片的小鼠子宫或进行了假手术的小鼠子宫的单核细胞群体进行了评估。带有宫内节育器碎片的子宫角明显比假手术的子宫角重。在使用铜宫内节育器的动物中，这种影响甚至延伸到未处理的对侧子宫角。带有宫内节育器的子宫角中的淋巴细胞总数明显高于假手术的子宫角。在未处理的对侧铜宫内节育器子宫角中也观察到了这一现象，但在硅橡胶宫内节育器处理动物的对侧子宫角中未观察到。在使用宫内节育器处理的动物和假手术动物之间，各种淋巴细胞群体的百分比以及绝对数量存在显著差异。同样，这种影响在使用铜宫内节育器处理的动物中更为明显，并在这些动物的对侧子宫角中也有体现。含有宫内节育器的子宫角中肥大细胞的数量也显著增加，铜宫内节育器在处理侧和对侧子宫角中再次产生了明显更显著的影响。假手术的小鼠两个子宫角的妊娠率均为67%。相比之下，使用宫内节育器处理的动物中，硅橡胶宫内节育器碎片处理侧和对侧子宫角的妊娠率显著降低（分别为15%和30%），而使用铜宫内节育器处理的动物（任一子宫角）的妊娠率为0%。
