International Joint Research Laboratory for Biointerface and Biodetection, State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, 214122, P. R. China.
Angew Chem Int Ed Engl. 2021 Jun 1;60(23):13073-13080. doi: 10.1002/anie.202103717. Epub 2021 May 6.
Hepatitis B virus (HBV) poses a severe threat to public health and social development. Here, we synthesized 4±0.5 nm copper (I) sulfide (Cu S) nanoparticles (NPs) with 46 mdeg chiroptical property at 530 nm to selectively cleavage HBV core antigen (HBcAg) and effectively blocked HBV assembly and prevented HBV infection both in vitro and in vivo under light at 808 nm. Experimental analysis showed that the chiral Cu S NPs specific bound with the functional domain from phenylalanine (F ) to leucine (L ) from HBcAg primary sequence and the cutting site was between amino acid residues F and proline (P ). Under excitation at 808 nm, the intracellular HBcAg concentration was reduced by 95 %, and in HBV transgenic mice, the levels of HBV surface antigen (HBsAg) and HBV DNA were decreased by 93 % and 86 %, respectively. Together, these results reveal the potential nanomedicine for HBV control and provide fresh tools for viral infection.
乙型肝炎病毒（HBV）对公共卫生和社会发展构成严重威胁。在这里，我们合成了具有 46 mdeg 手性光学特性的 4±0.5nm 铜（I）硫化物（CuS）纳米粒子（NPs），在 530nm 处选择性切割乙型肝炎核心抗原（HBcAg），并在 808nm 光下有效阻断了体外和体内的 HBV 组装和感染。实验分析表明，手性 CuS NPs 特异性结合 HBcAg 一级序列中从苯丙氨酸（F）到亮氨酸（L）的功能域，切割位点在氨基酸残基 F 和脯氨酸（P）之间。在 808nm 激发下，细胞内 HBcAg 浓度降低了 95%，在乙型肝炎转基因小鼠中，HBV 表面抗原（HBsAg）和 HBV DNA 的水平分别降低了 93%和 86%。综上所述，这些结果揭示了用于 HBV 控制的潜在纳米医学，并为病毒感染提供了新的工具。
