Antibacterial potency, cell viability and morphological implications of copper oxide nanoparticles encapsulated into cellulose acetate nanofibrous scaffolds.

It is generally believed that the most challenging impediment for the utilization of cellulose acetate (CA) in the medical field is its hydrophobicity and disability to poison the harmful microbes. Therefore, in this contribution, we aimed to prepare an environmentally scaffold-based CA loaded with copper nanoparticles (CuONPs), which are expected to not only improve the hydrophilicity of the prepared nanofibers, but also have an effective ability to kill such harmful and infectious microbes that are abundant in wounds. The obtained results attested that the generated nanofibers became thicker with increasing the content of CuONPs in CA nanofibers. The roughness average increased from 143.2 to 157.1 nm, whereas the maximum height of the roughness (R) increased from 400.8 to 479.9 nm as going from the lowest to the highest content of CuONPs. Additionally, the contact angle of the prepared nanofibers decreased from 105.3° (CA alone) to 85.4° for CuONPs@CA. Significantly, biological studies revealed that cell viability and anti-bacterial potency were improved upon incorporating CuONPs into CA solution. Correspondingly, their inhibition zones reached 18 ± 3 mm, and 16 ± 2 mm for nanofibrous scaffolds having 12.0CuO@CA, besides raising the cell viability from 91.3 ± 4% to 96.4 ± 4% for 0.0CuO@CA, and 12.0CuO@CA, respectively, thereby implying that the fabricated CuONPs@CA nanocomposite has biocompatibility towards fibroblast cells. Thus, introducing biological activity into CA nanofibers via loading with CuONPs makes it suitable for numerous biomedical applications, particularly as an environmentally benign wound dressing fibers.