Physics Department, Biophysics Branch, Faculty of Science, Ain Shams University, Cairo 11566, Egypt.
Nanomedicine Research Laboratories, Center for Materials Science, Zewail City of Science and Technology, 6 of October City 12578, Giza, Egypt.
Int J Biol Macromol. 2021 Dec 15;193(Pt B):2029-2037. doi: 10.1016/j.ijbiomac.2021.11.034. Epub 2021 Nov 11.
Every year, about 1 out of 9 get burnt in Egypt, with a mortality rate of 37%, and they suffer from physical disfigurement and trauma. For the treatment of second-degree burns, we aim at making a smart bandage provided with control of drug release (using chitosan nanoparticles) to enhance the healing process. This bandage is composed of natural materials; namely, cellulose acetate (CA), chitosan, and propolis (bee resin) as the loaded drug. Cellulose acetate nanofibers were deacetylated by NaOH after optimizing the reaction time and the concentration of NaOH solution, and the product was confirmed with FTIR analysis. Chitosan/propolis nanoparticles were prepared by ion gelation method with size ranging from 100 to 200 nm and a polydispersity index of 0.3. Chitosan/propolis nanoparticles were preloaded in the CA solution to ensure homogeneity. Loaded deacetylated cellulose nanofibers have shown the highest hydrophobicity measured by contact angle. Cytotoxicity of propolis and chitosan/propolis nanoparticles were tested and the experimental IC value was about 137.5 and 116.0 μg/mL, respectively, with p-value ≤0.001. In addition, chitosan/propolis nanoparticles loaded into cellulose nanofibers showed a cell viability of 89.46% in the cell viability test. In-vivo experiments showed that after 21 days of treatment with the loaded nanofibers repairing of epithelial cells, hair follicles and sebaceous glands in the skin of the burn wound were found in albino-mice model.
每年，埃及约有 1/9 的人被烧伤，死亡率为 37%，他们遭受身体毁容和创伤。为了治疗二度烧伤，我们的目标是制造一种带有药物释放控制功能的智能绷带（使用壳聚糖纳米粒子），以加速愈合过程。这种绷带由天然材料组成；即醋酸纤维素（CA）、壳聚糖和蜂胶（蜜蜂树脂）作为负载药物。通过优化反应时间和 NaOH 溶液的浓度，用 NaOH 对醋酸纤维素纳米纤维进行脱乙酰化处理，并用 FTIR 分析进行确认。壳聚糖/蜂胶纳米粒子采用离子凝胶化法制备，粒径为 100-200nm，多分散指数为 0.3。壳聚糖/蜂胶纳米粒子预先加载在 CA 溶液中以确保均匀性。负载脱乙酰化纤维素纳米纤维的疏水性最高，通过接触角测量。测试了蜂胶和壳聚糖/蜂胶纳米粒子的细胞毒性，实验 IC 值分别约为 137.5 和 116.0μg/ml，p 值均≤0.001。此外，壳聚糖/蜂胶纳米粒子负载到纤维素纳米纤维中，在细胞活力测试中显示出 89.46%的细胞活力。体内实验表明，在 21 天的治疗后，负载纳米纤维修复了烧伤皮肤的上皮细胞、毛囊和皮脂腺，在白化病小鼠模型中发现了这些细胞。
