Departamento de Bioquímica and Centro de Investigaciones Biomédicas (CENIBIO), Facultad de Medicina, Universidad de la República, Montevideo, Uruguay; Departamento de Nutrición Básica, Escuela de Nutrición, Universidad de la República, Montevideo, Uruguay.
Departamento de Bioquímica and Centro de Investigaciones Biomédicas (CENIBIO), Facultad de Medicina, Universidad de la República, Montevideo, Uruguay.
J Nutr Biochem. 2021 Aug;94:108646. doi: 10.1016/j.jnutbio.2021.108646. Epub 2021 Apr 7.
Non-alcoholic fatty liver disease (NAFLD) is characterized by excessive liver fat deposition in the absence of significant alcohol intake. Since extra virgin olive oil (EVOO) reduces fat accumulation, we analyzed the involvement of nitro-fatty acids (NO-FA) on the beneficial effects of EVOO consumption on NAFLD. Nitro-fatty acids formation was observed during digestion in mice supplemented with EVOO and nitrite. Mice fed with a high-fat diet (HF) presented lower plasma NO-FA levels than normal chow, and circulating concentrations recovered when the HF diet was supplemented with 10% EVOO plus nitrite. Under NO-FA formation conditions, liver hemoxygenase-1 expression significantly increased while decreased body weight and fat liver accumulation. Mitochondrial dysfunction plays a central role in the pathogenesis of NAFLD while NO-FA has been shown to protect from mitochondrial oxidative damage. Accordingly, an improvement of respiratory indexes was observed when mice were supplemented with both EVOO plus nitrite. Liver mitochondrial complexes II and V activities were greater in mice with EVOO supplementation and further improved in the presence of nitrite. Overall, our results strongly suggest a positive correlation between NO-OA formation from EVOO and the observed improvement of mitochondrial function in NAFLD. The formation of NO-FA can account for the health benefits associated with EVOO consumption.
非酒精性脂肪性肝病（NAFLD）的特征是在没有明显饮酒的情况下肝脏脂肪沉积过多。由于特级初榨橄榄油（EVOO）可减少脂肪堆积，因此我们分析了硝基脂肪酸（NO-FA）在 EVOO 消费对 NAFLD 的有益作用中的参与。在给小鼠补充 EVOO 和亚硝酸盐的消化过程中观察到了硝基脂肪酸的形成。与正常饲料相比，高脂肪饮食（HF）喂养的小鼠的血浆 NO-FA 水平较低，而当 HF 饮食补充 10% EVOO 和亚硝酸盐时，循环浓度恢复。在形成 NO-FA 的条件下，肝血红素加氧酶-1 的表达显著增加，而体重和脂肪肝堆积减少。线粒体功能障碍在 NAFLD 的发病机制中起着核心作用，而 NO-FA 已被证明可防止线粒体氧化损伤。因此，当给小鼠补充 EVOO 和亚硝酸盐时，观察到呼吸指数得到改善。EVOO 补充组的肝脏线粒体复合物 II 和 V 活性更高，在存在亚硝酸盐的情况下进一步提高。总的来说，我们的结果强烈表明，从 EVOO 形成的 NO-OA 与观察到的 NAFLD 中线粒体功能的改善之间存在正相关。NO-FA 的形成可以解释与 EVOO 消费相关的健康益处。
