Mohammadi-Ziveh Zahra, Mirhosseini Seyed Ali, Mahmoodzadeh Hosseini Hamideh
Applied Biotechnology Research Center, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
Department of Genetics, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
Iran J Pharm Res. 2020 Fall;19(4):169-180. doi: 10.22037/ijpr.2020.113275.14201.
Several formulations of herbal plants have been extensively applied to treat diseases. ( is an Iranian traditional plant with a wide range of benefit effects on different diseases. In this study, we aimed to prepare silver nanoparticles from via the green synthesis method and investigate the anti-cancer effects on the HT29 cell line. To synthesize Ag- 50 mL extract and 1 mM AgNO3 were mixed and shaken at room temperature for 72 h. To determine the Ag- nanoparticle characterization, XRD, FTIR, and TEM methods were done. In addition, MTT assay and real time PCR and annexin V/PI staining were performed to investigate the cytotoxicity, and gene expression and percentage of apoptotic cells. Our findings showed that Ag- is a spherical crystalline nanoparticle with the size less than 100 nm. MTT analysis showed that 375, 750, 1500 and 3000 µg/mL significantly decreased the cell viability of HT29 cells. Ag- significantly reduced and increased apoptotic index expression at 375, 750, 1500, 3000 µg/mL in a dose-dependent manner. Furthermore, cell staining with Annexin V/PI showed that treating Ag led to increasing in apoptotic cells. In conclusion, the formulation of Ag- has the apoptotic properties on the colorectal cancer cell line.
几种草药配方已被广泛应用于治疗疾病。（是一种伊朗传统植物，对不同疾病具有广泛的有益作用。在本研究中，我们旨在通过绿色合成方法从制备银纳米颗粒，并研究其对HT29细胞系的抗癌作用。为了合成Ag-，将50 mL提取物和1 mM硝酸银混合，并在室温下振荡72小时。为了确定Ag-纳米颗粒的特性，采用了XRD、FTIR和TEM方法。此外，进行MTT分析、实时PCR和膜联蛋白V/PI染色，以研究细胞毒性、基因表达和凋亡细胞百分比。我们的研究结果表明，Ag-是一种尺寸小于100 nm的球形晶体纳米颗粒。MTT分析表明，375、750、1500和3000 µg/mL的Ag-显著降低了HT29细胞的活力。Ag-在375、750、1500、3000 µg/mL时以剂量依赖性方式显著降低并增加凋亡指数表达。此外，用膜联蛋白V/PI对细胞进行染色表明，用Ag处理导致凋亡细胞增加。总之，Ag-制剂对结肠癌细胞系具有凋亡特性。
