Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran.
Mol Biol Rep. 2022 Feb;49(2):1113-1120. doi: 10.1007/s11033-021-06936-3. Epub 2021 Nov 24.
The present research was done to investigate the anticancer properties of silver nanoparticles (AgNPs) fabricated using bioactive extract of Onopordum acanthium L. (AgNPs-OAL) against breast cancer cells MDA_MB231 in vitro.
The determination studies of AgNPs-OAL were confirmed by X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM) analysis. Interestingly, the FESEM image observed the spherical shape of AgNPs-OAL with the range of 1-100 nm.
As AgNP-OAL exhibited significant cytotoxicity properties on breast cancer MDA_MB231 cells with IC values of 66.04 µg/mL, while lowing toxicity toward normal human embryonic kidney 293 (HEK293) cells with IC values of 101.04 µg/mL was evaluated. Further, up-regulation of apoptotic Bax and CAD gene expressions were confirmed by quantitative real-time reverse transcription-PCR (qRT-PCR) technique results. Moreover, enhanced cell cycle population (sub-G1), annexin V/PI staining, acridine orange and ethidium bromide (AO/EB) staining, Hoescht 33,258 dye, and generation of reactive oxygen species were observed in AgNP-OAL-treated MDA_MB231 cancer cells.
The green-synthesized AgNP-OAL has promising anticancer efficiency that can trigger apoptosis pathways in the MDA_MB231 breast cancer cells.
本研究旨在探讨使用黄花棘豆生物活性提取物制备的银纳米粒子（AgNPs-OAL）对体外乳腺癌 MDA_MB231 细胞的抗癌特性。
通过 X 射线衍射（XRD）、场发射扫描电子显微镜（FESEM）分析确认 AgNPs-OAL 的测定研究。有趣的是，FESEM 图像观察到 AgNPs-OAL 的球形形状，范围为 1-100nm。
AgNP-OAL 对乳腺癌 MDA_MB231 细胞表现出显著的细胞毒性，IC 值为 66.04µg/mL，而对正常人类胚胎肾 293（HEK293）细胞的毒性较低，IC 值为 101.04µg/mL。此外，通过定量实时逆转录聚合酶链反应（qRT-PCR）技术结果证实了凋亡 Bax 和 CAD 基因表达的上调。此外，在 AgNP-OAL 处理的 MDA_MB231 癌细胞中观察到细胞周期群体（sub-G1）增加、 Annexin V/PI 染色、吖啶橙和溴化乙锭（AO/EB）染色、Hoescht 33,258 染料和活性氧的产生。
AgNP-OAL 的绿色合成具有有前途的抗癌效率，可以在 MDA_MB231 乳腺癌细胞中触发细胞凋亡途径。
