Yu Furong, Liu Jie, Dong Weilei, Xie Jing, Zhao Xia
Department of Gynaecology and Obstetrics, The First Affiliated Hospital of University of South China Hengyang, Hu'nan Province, China.
Am J Transl Res. 2021 Mar 15;13(3):1825-1832. eCollection 2021.
To investigate the diagnostic value of miRNA-145 (miR-145) and miRNA-205 (miR-205) in cervical cancer patients.
Cervical tissue samples were collected from 144 patients diagnosed with and suspected to have cervical cancer in our hospital. Confirmed by pathology, 84 samples were obtained from cervical cancer patients and 60 samples were from patients with cervical intraepithelial neoplasia. Meanwhile, 30 patients with cervicitis were also selected, and the expression levels of miR-145, miR-205 and human papillomavirus (HPV) were detected in cervical lesions and normal cervical tissue.
In comparison to normal cervical tissue, cervicitis and cervical intraepithelial neoplasia groups, the relative expression level of miR-145 was significantly lower, whereas the relative expression level of miR-205 was notably higher in the cervical cancer group, respectively (P<0.001). The area under the receiver operating characteristic (ROC) curve of miR-145 for diagnosis of cervical cancer in patients was 0.878, of which the sensitivity and the specificity were 0.905 and 0.822, respectively. The area under the ROC curve of miR-205 was 0.881, of which the sensitivity and the specificity was 0.869 and 0.889, respectively. Among all patients, the relative expression level of miR-145 was significantly lower while the relative expression level of miR-205 was considerably higher in HPV-positive patients than those of HPV-negative groups (P<0.001). Parauterine invasion, FIGO stage III-IV and lymphatic metastasis were considered as independent factors that affect the expression of miR-145. FIGO stage III-IV and lymphatic metastasis were independent factors affecting the expression of miR-205.
The low expression level of miR-145 and the high expression level of miR-205 in patients with cervical cancer demonstrate a certain diagnostic value in cervical cancer. The expression level of miR-145 and miR-205 is correlated with HPV infection and cervical tumor malignancy.
探讨miRNA-145（miR-145）和miRNA-205（miR-205）在宫颈癌患者中的诊断价值。
收集我院144例诊断为及疑似患有宫颈癌的患者的宫颈组织样本。经病理确诊，84份样本来自宫颈癌患者，60份样本来自宫颈上皮内瘤变患者。同时，选取30例宫颈炎患者，检测宫颈病变组织及正常宫颈组织中miR-145、miR-205和人乳头瘤病毒（HPV）的表达水平。
与正常宫颈组织、宫颈炎和宫颈上皮内瘤变组相比，宫颈癌组中miR-145的相对表达水平显著降低，而miR-205的相对表达水平显著升高（P<0.001）。miR-145诊断宫颈癌患者的受试者工作特征（ROC）曲线下面积为0.878，其中敏感性和特异性分别为0.905和0.822。miR-205的ROC曲线下面积为0.881，其中敏感性和特异性分别为0.869和0.889。在所有患者中，HPV阳性患者的miR-145相对表达水平显著低于HPV阴性组，而miR-205的相对表达水平显著高于HPV阴性组（P<0.001）。子宫旁浸润、国际妇产科联盟（FIGO）分期III-IV期和淋巴转移被认为是影响miR-145表达的独立因素。FIGO分期III-IV期和淋巴转移是影响miR-205表达的独立因素。
宫颈癌患者中miR-145低表达和miR-205高表达在宫颈癌中具有一定的诊断价值。miR-145和miR-205的表达水平与HPV感染及宫颈肿瘤恶性程度相关。
