Beliavskaia Alexandra, Hönig Vaclav, Erhart Jan, Vyhlidalova Tereza, Palus Martin, Cerny Jiri, Kozlova Irina, Ruzek Daniel, Palomar Ana M, Bell-Sakyi Lesley
Department of Infection Biology and Microbiomes, Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, Liverpool, United Kingdom.
Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, Ceske Budejovice, Czechia.
Front Vet Sci. 2021 Mar 26;8:659786. doi: 10.3389/fvets.2021.659786. eCollection 2021.
are vertically-transmitted endosymbionts of ticks and other arthropods. Field-collected have been reported to harbour , but nothing is known about their persistence during laboratory colonisation of this tick species. We successfully isolated from internal organs of 6/10 unfed adult ticks, belonging to the third generation of an laboratory colony, into tick cell culture. We screened a further 51 adult male and female ticks from the same colony for presence of by genus-specific PCR amplification of fragments of the 16S rRNA and genes; 100% of these ticks were infected and the 16S rRNA sequence showed 99.8% similarity to that of a previously-published isolated from field-collected . Our study shows that endosymbionts persist at high prevalence in colonised through at least three generations, and confirms the usefulness of tick cell lines for isolation and cultivation of this bacterium.
是蜱虫和其他节肢动物的垂直传播内共生菌。据报道，野外采集的蜱虫携带该菌，但对于其在这种蜱虫实验室定殖过程中的持续性尚无了解。我们成功地从属于实验室群体第三代的10只未进食成年蜱虫中的6只的内部器官中分离出该菌，并将其接种到蜱虫细胞培养物中。我们通过对16S rRNA和基因片段进行属特异性PCR扩增，对来自同一群体的另外51只成年雌雄蜱虫进行了该菌存在情况的筛查；这些蜱虫100%被感染，且16S rRNA序列与先前从野外采集的蜱虫中分离出的该菌序列显示出99.8%的相似性。我们的研究表明，该内共生菌在定殖的蜱虫中至少三代都以高流行率持续存在，并证实了蜱虫细胞系对于分离和培养这种细菌的有用性。
