Department of Fish Diseases and Biology, Faculty of Veterinary Medicine, University of Life Sciences, Akademicka 12, 20-033 Lublin, Poland.
Department of Animal Biochemistry, Faculty of Veterinary Medicine, University of Life Sciences, Akademicka 12, 20-033 Lublin, Poland.
Pol J Vet Sci. 2021 Mar;24(1):79-84. doi: 10.24425/pjvs.2021.136795.
Essential oils from plants used in traditional medicine are known as a rich source of chemically diverse compounds with specific biological activities. Achillea millefolium essential oil (AEO) was screened for in vitro activity against Babesia canis. The AEO was obtained by hydrodistillation and analysed by gas chromatography coupled to mass spectrometry (GC-MS). GC-MS revealed the presence of 47 compounds in the essential oil. Those present in the highest concentrations were chamazulene (34.45%), β-caryophyllene (8.93%), (E)-germacrene D (7.55%), patchoulene (7.27%), β-guaiene (4.62%), α-humulene (4.59%), santolina epoxide (4.41%), ethyl iso-allocholate (2.97%), aromadendrene (2.62%), and neoclovenoxid-alkohol (2.46%). AEO was found to be active in vitro against B. canis, with 50% inhibitory concentration (IC50) values of 0.06 mg/mL, as compared to imidocarb, with IC50 = 0.007 mg/mL. The study confirms that essential oil from A. millefolium has anti-babesial properties in vitro.
来自传统医学中使用的植物的精油被认为是具有特定生物活性的化学多样性化合物的丰富来源。对紫锥菊精油（AEO）进行了体外抗犬巴贝斯虫活性筛选。AEO 通过水蒸馏获得，并通过气相色谱-质谱联用仪（GC-MS）进行分析。GC-MS 显示精油中存在 47 种化合物。含量最高的化合物为天蓝烃（34.45%）、β-石竹烯（8.93%）、（E）-金合欢烯 D（7.55%）、广藿香烯（7.27%）、β-愈创木烯（4.62%）、α-葎草烯（4.59%）、桑托里烯氧化物（4.41%）、异戊酸乙酯（2.97%）、芳樟醇（2.62%）和新扣烷氧化醇（2.46%）。研究表明，A. millefolium 精油对 B. canis 具有体外抗巴贝斯虫活性，50%抑制浓度（IC50）值为 0.06mg/mL，而咪多卡的 IC50 值为 0.007mg/mL。该研究证实，紫锥菊精油具有体外抗巴贝斯虫的特性。
