Guz L, Adaszek Ł, Wawrzykowski J, Ziętek J, Winiarczyk S
Department of Fish Diseases & Biology, Faculty of Veterinary Medicine, University of Life Sciences, Akademicka 12, 20-033 Lublin, Poland.
Department of Epizootiology and Clinic of Infectious Diseases, Faculty of Veterinary Medicine, University of Life Sciences, Głęboka 30, 20-612 Lublin, Poland.
Pol J Vet Sci. 2019 Jun;22(2):369-376. doi: 10.24425/pjvs.2019.129230.
Despite many phytochemical and pharmacological investigations, to date, there are no reports concerning the antibabesial activity of extracts of A. millefolium against B. canis. This study was aimed at investigating the biological activities of A. millefolium against the Babesia canis parasite and to identify its chemical ingredients. The water (WE), ethanol (EE) and hexane/acetone (H/AE) extracts of plant aerial parts were screened for total phenolic content (TPC), total flavonoid compound (TFC), DPPH free radical-scavenging activity and its antibabesial activity assay. In this study, imidocarb diproprionate was used as a positive control. The H/AE and EE extracts were analysed using gas chromatography-mass spectroscopy (GC-MS). In the EE extract, the main compounds were 17.64% methyl octadec-9-ynoate, 16.68% stigmast-5-en-3-ol(3α,24S) and 15.17% hexadecanoic acid. In the H/AE extract, the main compounds were 34.55% 11-decyldocosane, 14.31% N-tetratetracontane, 8.22% β-caryophyllene, and 7.69% N-nonacosane. Extract of EE contained the highest content of phenolics followed by H/AE and WE. The concentration of flavonoids in EE, H/AE and WE extracts showed that TFC was higher in the EE samples followed by H/AE and WE. The antioxidant activities were highest for AA, followed by EE, WE and H/AE. The antibabesial assay showed that the WE, EE and H/AE extracts of A. millefolium were antagonistic to B. canis. At a 2 mg/mL concentration, it showed 58.7% (± 4.7%), 62.3% (± 5.5%) and 49.3% (± 5.1%) inhibitory rate in an antibabesial assay, respectively. Considering these results, the present findings suggest that A. millefolium extracts may be a potential therapeutic agent and that additional studies including in vivo experiments are essential.
尽管进行了许多植物化学和药理学研究，但迄今为止，尚无关于千叶蓍提取物对犬巴贝斯虫的抗巴贝斯虫活性的报道。本研究旨在研究千叶蓍对犬巴贝斯虫寄生虫的生物活性，并鉴定其化学成分。对植物地上部分的水提取物（WE）、乙醇提取物（EE）和己烷/丙酮提取物（H/AE）进行了总酚含量（TPC）、总黄酮化合物（TFC）、DPPH自由基清除活性及其抗巴贝斯虫活性测定。在本研究中，双丙酸咪唑苯脲用作阳性对照。使用气相色谱-质谱联用仪（GC-MS）对EE提取物和H/AE提取物进行分析。在EE提取物中，主要化合物为17.64%的9-十八碳炔酸甲酯、16.68%的豆甾-5-烯-3-醇（3α，24S）和15.17%的十六烷酸。在H/AE提取物中，主要化合物为34.55%的11-癸基二十二烷、14.31%的正四十四烷、8.22%的β-石竹烯和7.69%的正二十九烷。EE提取物中酚类含量最高，其次是H/AE提取物和WE提取物。EE提取物、H/AE提取物和WE提取物中的黄酮类化合物浓度表明，EE样品中的TFC含量最高，其次是H/AE提取物和WE提取物。抗氧化活性以AA最高，其次是EE提取物、WE提取物和H/AE提取物。抗巴贝斯虫试验表明，千叶蓍的WE提取物、EE提取物和H/AE提取物对犬巴贝斯虫具有拮抗作用。在2 mg/mL浓度下，其在抗巴贝斯虫试验中的抑制率分别为58.7%（±4.7%）、62.3%（±5.5%）和49.3%（±5.1%）。考虑到这些结果，目前的研究结果表明千叶蓍提取物可能是一种潜在的治疗剂，包括体内实验在内的进一步研究至关重要。
