Department of Physics, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China.
3rd Institute of Physics and Center for Applied Quantum Technologies, University of Stuttgart, 70569 Stuttgart, Germany.
Nano Lett. 2021 Apr 28;21(8):3393-3400. doi: 10.1021/acs.nanolett.0c04864. Epub 2021 Apr 13.
Correlated translation-orientation tracking of single particles can provide important information for understanding the dynamics of live systems and their interaction with the probes. However, full six-dimensional (6D) motion tracking has yet to be achieved. Here, we developed synchronized 3D translation and 3D rotation tracking of single diamond particles based on nitrogen-vacancy center sensing. We first performed 6D tracking of diamond particles attached to a giant plasma membrane vesicle to demonstrate the method. Quantitative analysis of diamond particles' motion allowed elimination of the geometric effect and revealed the net rotation on the vesicle. 6D tracking was then applied to measure live cell dynamics. Motion characteristics of nanodiamonds on cell membranes under various controlled physiological conditions suggest that the nanodiamonds' rotation is associated with cell metabolic activities. Our technique extends the toolbox of single particle tracking and provides a unique solution to problems where correlated analysis of translation and rotation is critical.
关联的单颗粒追踪可以为理解活系统的动力学及其与探针的相互作用提供重要信息。然而，全六维（6D）运动追踪尚未实现。在这里，我们基于氮空位中心传感开发了单金刚石颗粒的同步三维平移和三维旋转追踪。我们首先对附在巨大质膜囊泡上的金刚石颗粒进行了 6D 追踪，以验证该方法。对金刚石颗粒运动的定量分析消除了几何效应，并揭示了囊泡上的净旋转。然后将 6D 追踪应用于测量活细胞动力学。在各种受控生理条件下，细胞膜上纳米金刚石的运动特征表明纳米金刚石的旋转与细胞代谢活动有关。我们的技术扩展了单颗粒追踪工具包，并为关联分析平移和旋转至关重要的问题提供了独特的解决方案。
