Okada J, Higuchi I, Kondo S, Saito B
Takeda Chemical Industries, Ltd, Research Laboratories, Animal Health Products Division, Osaka, Japan.
J Assoc Off Anal Chem. 1988 Mar-Apr;71(2):337-40.
A paper disc method is described for determination of residual cephalexin (CEX) in chick tissues. A trichloroacetic acid extract of plasma and tissues is chromatographed on a macroreticular resin (Diaion HP-20) column to remove endogenous antibacterial substances interfering with the assay. The eluate is evaporated to dryness and the residue, dissolved in methanol-water (1 + 2), is subjected to a paper disc assay using Bacillus stearothermophilus var. calido-lactis C953 NIZO as a test organism. The detection limit was 0.0375 ppm in tissue; the average recovery of CEX ranged from 72.4% in skin to 90.4% in plasma. Water containing 200 or 500 mg/L of CEX was given ad libitum to 2-week-old chicks for 10 days; the highest levels of CEX were found in the kidney, and the lowest were found in muscle at 0 h of withdrawal. CEX disappeared from most tissues at 24 h after withdrawal except from skin of chicks given 500 mg/L. However, the drug was not detected in the skin at 48 h after withdrawal.
本文描述了一种用于测定鸡组织中残留头孢氨苄（CEX）的纸碟法。血浆和组织的三氯乙酸提取物在大孔网状树脂（Diaion HP - 20）柱上进行色谱分离，以去除干扰测定的内源性抗菌物质。洗脱液蒸发至干，残留物溶解于甲醇 - 水（1 + 2）中，使用嗜热脂肪芽孢杆菌变种嗜热乳酸C953 NIZO作为测试菌进行纸碟法测定。组织中的检测限为0.0375 ppm；CEX的平均回收率在皮肤中为72.4%，在血浆中为90.4%。将含有200或500 mg/L CEX的水随意提供给2周龄的雏鸡，持续10天；停药0小时时，肾脏中CEX水平最高，肌肉中最低。停药后24小时，除给予500 mg/L的雏鸡皮肤外，大多数组织中的CEX消失。然而，停药48小时后在皮肤中未检测到该药物。
