Meissner J, Pfennig N, Krauss J H, Mayer H, Weckesser J
Institut für Biologie II, Albert-Ludwigs-Universität, Freiburg im Breisgau, Federal Republic of Germany.
J Bacteriol. 1988 Jul;170(7):3217-22. doi: 10.1128/jb.170.7.3217-3222.1988.
The lipopolysaccharides (LPS) of three species of purple sulfur bacteria (Chromatiaceae), Thiocystis violacea, Thiocapsa pfennigii, and the moderately thermophilic bacterium Chromatium tepidum, were isolated. The LPS of Thiocystis violacea and Chromatium tepidum contained typical O-specific sugars, indicating O-chains. Long O-chains were confirmed for these species by sodium deoxycholate gel electrophoresis of their LPS. Thiocapsa pfennigii, however, had short or no O-chains. The core region of the LPS of all three species comprised D-glycero-D-mannoheptose as the only heptose and 2-keto-3-deoxyoctonate. The lipid A, obtained from the LPS by mild acid hydrolysis, contained glucosamine as the main amino sugar. Amide-bound 3-hydroxymyristic acid was the only hydroxy fatty acid. The main ester-bound fatty acid in all lipid A fractions was 12:0. Mannose and small amounts of 2,3-diamino-2,3-dideoxy-D-glucose were common constituents of the lipid A of the three Chromatiaceae species investigated. All lipid A fractions were essentially free of phosphate.
分离出了三种紫色硫细菌（着色菌科）——紫硫囊菌、芬氏硫囊菌和嗜温嗜色菌的脂多糖（LPS）。紫硫囊菌和嗜温嗜色菌的LPS含有典型的O-特异性糖，表明存在O-链。通过对其LPS进行脱氧胆酸钠凝胶电泳，证实了这些菌种具有长O-链。然而，芬氏硫囊菌的O-链短或没有O-链。所有这三个菌种的LPS核心区域都含有D-甘油-D-甘露庚糖作为唯一的庚糖和2-酮-3-脱氧辛酸。通过温和酸水解从LPS获得的类脂A含有葡糖胺作为主要氨基糖。酰胺结合的3-羟基肉豆蔻酸是唯一的羟基脂肪酸。所有类脂A组分中的主要酯结合脂肪酸是12:0。甘露糖和少量的2,3-二氨基-2,3-二脱氧-D-葡萄糖是所研究的三种着色菌科菌种类脂A的常见成分。所有类脂A组分基本不含磷酸盐。
