Hurlbert R E, Weckesser J, Tharanathan R N, Mayer H
Eur J Biochem. 1978 Oct;90(2):241-6. doi: 10.1111/j.1432-1033.1978.tb12596.x.
The lipopolysaccharide from Thiocapsa roseopersicina was isolated by phenol/water, being found in the water phase. It is cleaved into a polysaccharide moiety (degraded polysaccharide) and lipid A by hydrolysis with 10% acetic acid (100 degree C, 3 h). D-Mannose, L-rhamnose, 3-amino-3, 6-dideoxy-D-galactose and D-glucose are the major constituents of the degraded polysaccharide. 2-O-Methyl-L-rhamnose, 3-O-methyl-D-mannose, D-galactose, glucosamine and quinovosamine are minor constituents. D-Glycer-D-manno-heptose (tentatively identified) and 3-deoxy-D-manno-octulosonic acid were detected in only small amounts. Conspicuously, lipid A from T. roseopersicina contains a neutral sugar, D-mannose, in addition to D-glucosamine, as had been observed with lipid A from Chromatium vinosum D. Major fatty acids are beta-hydroxymyristic and lauric acids. Only trace amounts of phosphorus were found indicating this lipid A to be free of phosphate. The lipopolysaccharide of T. roseopersicina represents the O-antigen of the strain. It reacts with antisera prepared against living or heat-killed cells in passive hemagglutination.
从玫瑰色硫囊菌中分离出的脂多糖通过苯酚/水法提取，存在于水相中。用10%乙酸（100℃，3小时）水解可将其裂解为多糖部分（降解多糖）和脂质A。D-甘露糖、L-鼠李糖、3-氨基-3,6-二脱氧-D-半乳糖和D-葡萄糖是降解多糖的主要成分。2-O-甲基-L-鼠李糖、3-O-甲基-D-甘露糖、D-半乳糖、氨基葡萄糖和奎诺糖胺是次要成分。仅检测到少量的D-甘油-D-甘露庚糖（初步鉴定）和3-脱氧-D-甘露辛酮酸。值得注意的是，玫瑰色硫囊菌的脂质A除了含有D-氨基葡萄糖外，还含有一种中性糖D-甘露糖，这与嗜硫红假单胞菌D的脂质A情况相同。主要脂肪酸是β-羟基肉豆蔻酸和月桂酸。仅发现痕量的磷，表明这种脂质A不含磷酸盐。玫瑰色硫囊菌的脂多糖代表该菌株的O抗原。它在被动血凝反应中与针对活细胞或热灭活细胞制备的抗血清发生反应。
