评估柑橘属柠檬（Citrus aurantium L.）对乙酰胆碱酯酶活性和淀粉样纳米生物纤维产生的抗氧化和抑制特性。

Evaluation of antioxidant and inhibitory properties of Citrus aurantium L. on the acetylcholinesterase activity and the production of amyloid nano-bio fibrils.

机构信息

Department of Biology, Rasht Branch, Islamic Azad University, Rasht, Iran.

出版信息

Int J Biol Macromol. 2021 Jul 1;182:366-372. doi: 10.1016/j.ijbiomac.2021.04.043. Epub 2021 Apr 14.

DOI:10.1016/j.ijbiomac.2021.04.043

Abstract

The blossoms of Citrus aurantium are considered for the treatment of Alzheimer's disease because of their fragrant essential oils. The aim of this study was to investigate the antioxidant and inhibitory effects of Citrus aurantium extract on the acetylcholinesterase and production of amyloid nanobiofibrils from bovine serum albumin (BSA). The Citrus aurantium petals were harvested from Rasht city in northern IRAN. Chemical composition was investigated by GC-MS. The anti-Alzheimer's effects were evaluated by determining the antioxidant percentage by DPPH method and determining acetylcholinesterase activity. Congored spectroscopy was used for investigation of the inhibitory properties of the extract on the production of amyloid nanobiofibrils, and amyloid fibers was confirmed by electron microscopy. The most abundant ingredients were D-Glucuronic acid (9.53%), D-Limonene (5.54%), Linalool (2.06%), Daphnetin (3.73%), Phthalic acid (0.72%), Octadecenoic acid (3.98%), Hexadecanoic acid (2.13%), Pyrrolidinone (1.17%) and the highest antioxidant capacity was at 8 mg/ml (EC: 2.36 mg/ml). The extract reduced the Acetylcholinesterase activity less than 47.04% (IC: 42.8 mg/ml) and amyloid production less than 22% (EC: 3.135). Citrus aurantium petals with inhibitory properties for the production of amyloid nanobiofibrils, can be used as a beneficial drugs for reducing side effects of Alzheimer's disease.

摘要

柑橘属植物的花朵因其芳香精油而被认为可用于治疗阿尔茨海默病。本研究旨在研究柑橘属植物提取物对乙酰胆碱酯酶的抗氧化和抑制作用，以及对牛血清白蛋白（BSA）产生的淀粉样纳米生物纤维的抑制作用。从伊朗北部拉什特市收获了柑橘属植物的花瓣。通过 GC-MS 研究了化学成分。通过 DPPH 法测定抗氧化百分比和测定乙酰胆碱酯酶活性来评估抗阿尔茨海默病作用。使用刚果红光谱法研究提取物对淀粉样纳米生物纤维产生的抑制特性，并通过电子显微镜证实了淀粉样纤维的存在。最丰富的成分是 D-葡萄糖醛酸（9.53％），D-柠檬烯（5.54％），芳樟醇（2.06％），瑞香素（3.73％），邻苯二甲酸（0.72％），十八碳烯酸（3.98％），十六烷酸（2.13％），吡咯烷酮（1.17％），抗氧化能力最高的浓度为 8mg/ml（EC：2.36mg/ml）。该提取物使乙酰胆碱酯酶活性降低小于 47.04％（IC：42.8mg/ml），并使淀粉样蛋白产生降低小于 22％（EC：3.135）。具有抑制淀粉样纳米生物纤维产生特性的柑橘属植物花瓣，可用作减少阿尔茨海默病副作用的有益药物。