Graham L, Warren N G, Tsang A Y, Dalton H P
Department of Pathology, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298.
J Clin Microbiol. 1988 May;26(5):1034-6. doi: 10.1128/jcm.26.5.1034-1036.1988.
From July 1983 through November 1985, organisms belonging to Mycobacterium avium complex were isolated from the urine of 29 patients. Strains recovered from the urine of nine patients from July 1983 through August 1984 were serotyped. Eight of the nine samples belonged to serovar 4. M. avium complex was isolated from the urine of 21 patients during the period from November 1984 through November 1985. While the possibility of a point source contamination was investigated, M. avium complex was recovered from the phenol red solution used for processing urine specimens in the mycobacteriology laboratory and the deionized tap water of that laboratory that is used to make the reagent. M. avium complex serovar 4 was subsequently recovered from the tap water of the laboratory and four hospital wards. During the year following the installation of a microbiological filter for the mycobacteriology laboratory deionized tap water, 2 urine isolates were recovered, compared to 26 the previous year. This study demonstrates the importance of filtration devices at tap water sites that are used to make laboratory reagents and the value of serotyping as a marker for the detection of a specific source of M. avium complex contamination.
从1983年7月至1985年11月,从29例患者的尿液中分离出鸟分枝杆菌复合群的微生物。对1983年7月至1984年8月期间从9例患者尿液中分离出的菌株进行了血清分型。9个样本中有8个属于血清型4。在1984年11月至1985年11月期间,从21例患者的尿液中分离出鸟分枝杆菌复合群。在调查是否存在点源污染的过程中,从用于处理分枝杆菌实验室尿液标本的酚红溶液以及该实验室用于制备试剂的去离子自来水中分离出了鸟分枝杆菌复合群。随后从该实验室的自来水和四个医院病房中分离出了血清型4的鸟分枝杆菌复合群。在为分枝杆菌实验室去离子自来水安装微生物过滤器后的一年中,分离出2株尿液菌株,而前一年为26株。本研究证明了用于制备实验室试剂的自来水处过滤装置的重要性以及血清分型作为检测鸟分枝杆菌复合群特定污染源标志物的价值。