Thoresen O F, Saxegaard F
National Veterinary Institute, Oslo, Norway.
Vet Microbiol. 1993 Jan;34(1):83-8. doi: 10.1016/0378-1135(93)90009-v.
Commercially available DNA probes for the Mycobacterium avium complex (MAC) were compared with conventional identification and serotyping of animal isolates of MAC. DNA hybridization of 44 strains of mycobacteria showed a test specificity of 100% and sensitivity of 100%. Hybridization of 12 serotype strains showed that serotypes 1-6 and 8-11 hybridized with the M. avium probe and serotypes 7 and 14 hybridized with the M. intracellulare probe. All of the 42 bovine and porcine isolates of MAC consisting of serotypes 1-6 and 8-10 hybridized only with the M. avium probe. Two strains originally identified as MAC by biochemical tests turned out to be negative in the hybridization test and were identified as rapid growers in gas chromatography analysis of fatty acids. This study furthermore indicates that MAC infections in animals in Norway are mainly caused by M. avium probe positive strains.
将市售的鸟分枝杆菌复合群（MAC）DNA探针与动物分离株MAC的传统鉴定和血清分型方法进行了比较。对44株分枝杆菌进行DNA杂交，结果显示检测特异性为100%，敏感性为100%。对12株血清型菌株进行杂交，结果表明血清型1 - 6和8 - 11与鸟分枝杆菌探针杂交，血清型7和14与胞内分枝杆菌探针杂交。42株由血清型1 - 6和8 - 10组成的牛和猪MAC分离株仅与鸟分枝杆菌探针杂交。最初通过生化试验鉴定为MAC的两株菌株在杂交试验中结果为阴性，在脂肪酸气相色谱分析中被鉴定为快速生长菌。本研究还表明，挪威动物中的MAC感染主要由鸟分枝杆菌探针阳性菌株引起。
