用于检测脑源性神经营养因子Val66Met多态性的硅基铝微梳电极
Aluminum Microcomb Electrodes on Silicon Wafer for Detecting Val66Met Polymorphism in Brain-Derived Neurotrophic Factor.
作者信息
Li Zhi, Cui Liangmin, Zhao Hongyao, Du Jinxin, Gopinath Subash C B, Lakshmipriya Thangavel, Xin Xuezhi
机构信息
Department of General Surgery, The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, Jinan, China.
Key Laboratory of Metabolism and Gastrointestinal Tumor, the First Affiliated Hospital of Shandong First Medical University, Jinan, China.
出版信息
Dev Neurosci. 2021;43(1):53-62. doi: 10.1159/000515197. Epub 2021 Apr 13.
OBJECTIVE
Brain-derived neurotrophic factor (BDNF) dysregulation is widely related with various psychiatric and neurological disorders, including schizophrenia, depression, Rett syndrome, and addiction, and the available evidence suggests that BDNF is also highly correlated with Parkinson's and Alzheimer's diseases.
METHODS
The BDNF target sequence was detected on a capture probe attached on aluminum microcomb electrodes on the silicon wafer surface. A capture-target-reporter sandwich-type assay was performed to enhance the detection of the BDNF target.
RESULTS
The limit of detection was noticed to be 100 aM. Input of a reporter sequence at concentrations >10 aM improved the detection of the target sequence by enhancing changes in the generated currents. Control experiments with noncomplementary and single- and triple-mismatches of target and reporter sequences did not elicit changes in current levels, indicating the selective detection of the BDNF gene sequence.
CONCLUSION
The above detection strategy will be useful for the detection and quantification of BDNF, thereby aiding in the provision of suitable treatments for BDNF-related disorders.
目的
脑源性神经营养因子（BDNF）失调与多种精神和神经疾病广泛相关，包括精神分裂症、抑郁症、雷特综合征和成瘾，现有证据表明BDNF也与帕金森病和阿尔茨海默病高度相关。
方法
在附着于硅片表面铝微梳电极上的捕获探针上检测BDNF靶序列。进行捕获-靶标-报告分子夹心型检测以增强对BDNF靶标的检测。
结果
检测限为100 aM。浓度>10 aM的报告序列输入通过增强产生电流的变化改善了靶序列的检测。用非互补以及靶标和报告序列的单错配和三错配进行的对照实验未引起电流水平变化，表明对BDNF基因序列的选择性检测。
结论
上述检测策略将有助于BDNF的检测和定量，从而有助于为BDNF相关疾病提供合适的治疗方法。
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