Woods T C, McKinney R M, Plikaytis B D, Steigerwalt A G, Bibb W F, Brenner D J
Immunology Laboratory, Centers for Disease Control, Atlanta, Georgia 30333.
J Clin Microbiol. 1988 May;26(5):799-803. doi: 10.1128/jcm.26.5.799-803.1988.
Variability among 29 clinical and environmental strains of Legionella dumoffii was investigated by multilocus enzyme analysis by use of starch gel electrophoresis. Based on results of analysis at 20 enzyme loci, the strains were separated into five closely related electrophoretic types (ETs), which were clearly distinguished from 53 strains representing 53 ETs of L. pneumophila. DNA hybridization results (hydroxyapatite method, 60 and 75 degrees C) for representative strains confirmed that all L. dumoffii ETs were a single genetic species. Although multilocus enzyme analysis indicated that L. dumoffii was genetically a quite uniform species, sufficient variability existed to warrant electromorph fingerprinting for epidemiologic studies.
通过使用淀粉凝胶电泳的多位点酶分析，研究了29株杜莫夫军团菌临床菌株和环境菌株之间的变异性。基于20个酶位点的分析结果，这些菌株被分为五个密切相关的电泳类型（ETs），它们与代表嗜肺军团菌53个ETs的53株菌株明显区分开来。代表性菌株的DNA杂交结果（羟基磷灰石法，60和75摄氏度）证实，所有杜莫夫军团菌ETs均为单一遗传物种。尽管多位点酶分析表明杜莫夫军团菌在遗传上是一个相当均匀的物种，但仍存在足够的变异性，足以保证在流行病学研究中进行电形态指纹分析。
