Woods T C, Helsel L O, Swaminathan B, Bibb W F, Pinner R W, Gellin B G, Collin S F, Waterman S H, Reeves M W, Brenner D J
Meningitis and Special Pathogens Branch, Centers for Disease Control, Atlanta, Georgia 30333.
J Clin Microbiol. 1992 Jan;30(1):132-7. doi: 10.1128/jcm.30.1.132-137.1992.
We compared multilocus enzyme electrophoresis (MEE) and ribosomal DNA fingerprinting (ribotyping) for subtyping 44 strains of Neisseria meningitidis serogroup C that were isolated in Los Angeles County, California, between December 1985 and July 1986. The isolates were divided into six enzyme types (ETs) by MEE, but 36 of the isolates were clustered in one ET, 3. The same isolates were divided into 17 ribotypes by use of restriction endonucleases ClaI, EcoRI, and XhoI. Twenty of the 36 ET 3 isolates were divided into 17 ribotypes by use of restriction endonucleases ClaI, EcoRI, and XhoI. Twenty of the 36 ET 3 isolates were grouped in a single ribotype, J. The rate of infection with ribotype J strains was higher in the southern part of the study area than in the northern part. Isolates from each of eight pairs (each isolate pair was cultured from the same patient from the same or different sites) were found identical by MEE, but ribotyping revealed a difference in one pair. In this study, ribotyping showed a greater discriminating capacity than MEE for subtyping N. meningitidis serogroup C, but the epidemiologic relevance of this increased sensitivity needs further assessment.
我们比较了多位点酶电泳（MEE）和核糖体DNA指纹图谱分析（核糖体分型）对1985年12月至1986年7月间在加利福尼亚州洛杉矶县分离出的44株C群脑膜炎奈瑟菌进行亚型分型的效果。通过MEE将分离株分为六种酶型（ETs），但其中36株分离株聚集在一种酶型，即酶型3中。使用限制性内切酶ClaI、EcoRI和XhoI将相同的分离株分为17种核糖体分型。36株酶型3分离株中的20株通过使用限制性内切酶ClaI、EcoRI和XhoI被分为17种核糖体分型。36株酶型3分离株中的20株被归为单一的核糖体分型J。核糖体分型J菌株在研究区域南部的感染率高于北部。通过MEE发现来自八对菌株（每对菌株均从同一患者的相同或不同部位培养获得）中的每一对的分离株均相同，但核糖体分型显示其中一对存在差异。在本研究中，对于C群脑膜炎奈瑟菌的亚型分型，核糖体分型比MEE具有更强的鉴别能力，但这种更高敏感性的流行病学相关性需要进一步评估。
