Smith H J, Moore H B
Department of Biology, San Diego State University, California 92182.
J Clin Microbiol. 1988 Jun;26(6):1134-7. doi: 10.1128/jcm.26.6.1134-1137.1988.
New and selective Rlk and SA media, combined with cold enrichment at 4 to 5 degrees C, allowed isolation of Mobiluncus species from patients with bacterial vaginosis at higher rates than with conventional cultivation methods. Rlk medium consists of Columbia CNA agar supplemented with peptone, yeast extract, 5% laked rabbit or sheep blood, nalidixic acid, and tinidazole. SA medium consists of Columbia CNA agar supplemented with 2% rabbit serum, 1.6% laked rabbit or sheep blood, nalidixic acid, and tinidazole. Use of these selective media plus the cold enrichment technique permitted Mobiluncus species to propagate at rates similar to those of other anaerobic members of the vaginal flora.
新型选择性Rlk培养基和SA培养基，结合4至5摄氏度的冷增菌法，能比传统培养方法以更高的比率从细菌性阴道病患者中分离出动弯杆菌属菌种。Rlk培养基由添加了蛋白胨、酵母提取物、5%脱纤维兔血或羊血、萘啶酸和替硝唑的哥伦比亚CNA琼脂组成。SA培养基由添加了2%兔血清、1.6%脱纤维兔血或羊血、萘啶酸和替硝唑的哥伦比亚CNA琼脂组成。使用这些选择性培养基加上冷增菌技术能使动弯杆菌属菌种以与阴道菌群中其他厌氧菌相似的速率繁殖。
