Centre for Nanosciences and Molecular Medicine, Amrita Vishwa Vidyapeetham, Kochi, India.
Department of Gynecological Oncology, Amrita Institute of Medical Sciences and Research Centre, Amrita Vishwa Vidyapeetham, Kochi, India.
J Med Virol. 2021 Aug;93(8):5188-5192. doi: 10.1002/jmv.27015. Epub 2021 Apr 23.
The prevalence of human papillomavirus (HPV) types varies geographically between various countries and different parts of the same country. The efficacy of the HPV vaccines is dependent on the prevalent HPV types. Here, we have studied the prevalence of high-risk HPV (hrHPV) and its genotypes in women in our population. Cervical samples of 2443 women were screened for the presence of hrHPV using the careHPV system. To determine the HPV genotypes, viral DNA was isolated from the hrHPV-positive samples, nested PCR was used to amplify the L1 hypervariable region, and was subjected to Sanger sequencing. The prevalence of hrHPV was found to be 2%. HPV16 (52%), HPV33 (40%), HPV18 (4%), HPV31 (2%), and HPV66 (2%) genotypes were found in this study. In Kerala, HPV16 and HPV33 genotypes were found to be significantly higher compared with the other HPV types detected. As the bivalent (Cervarix) and quadrivalent (Gardasil-4) vaccines offer limited cross-protection against HPV33, nonavalent (Gardasil 9) vaccine would be more effective in preventing cervical carcinoma in Kerala.
人乳头瘤病毒（HPV）的流行率在不同国家和同一国家的不同地区存在差异。HPV 疫苗的功效取决于流行的 HPV 类型。在这里，我们研究了我们人群中女性高危型 HPV（hrHPV）及其基因型的流行率。使用 careHPV 系统对 2443 名女性的宫颈样本进行了 hrHPV 检测。为了确定 HPV 基因型，从 hrHPV 阳性样本中分离出病毒 DNA，使用巢式 PCR 扩增 L1 高变区，并进行 Sanger 测序。结果发现，hrHPV 的流行率为 2%。在这项研究中发现 HPV16（52%）、HPV33（40%）、HPV18（4%）、HPV31（2%）和 HPV66（2%）基因型。在喀拉拉邦，HPV16 和 HPV33 基因型的检出率明显高于其他 HPV 类型。由于二价（Cervarix）和四价（Gardasil-4）疫苗对 HPV33 提供的交叉保护有限，因此九价（Gardasil 9）疫苗在喀拉拉邦预防宫颈癌的效果会更好。
