School of Food and Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu Province 212013, People's Republic of China.
Department of Botany and Microbiology, Faculty of Science, Alexandria University, Alexandria 21521, Egypt.
Nanotechnology. 2021 Jun 21;32(37). doi: 10.1088/1361-6528/abf7ee.
Despite the major medical advancements in recent decades, treating infected wounds successfully remains a challenge. In this research, a functional blend of Polyhydroxybutyrate (PHB) and Chitosan (Cs) was developed for wound infection mitigation with tailored biological and physicochemical properties. Water insoluble kaempferol (KPF) was pre-formulated to water soluble KPF nanocrystals (KPF-NCs) with fine particle size of 145 ± 11 nm, and high colloidal stability (-31 ± 0.4 mV) to improve its drug transdermal delivery. PHB-Cs-KPF-NCs (1:2 ratio) film owned the best physical properties in terms of high breathability, thermal stability and mechanical strength (33 ± 1 MPa). Besides, XRD and FTIR findings indicated the interaction between Cs, PHB and KPF, reducing the film crystallinity. The scanning electron microscopy of the film displayed a highly interconnected porous morphology. KPF-NCs were integrated in PHB-Cs matrix with a marked encapsulation efficiency of 96.6%. The enhanced drug-loading film showed a sustain release pattern of KPF-NCs over 48 h. Interestingly, the developed blend possessed an impressive blood clotting capacity within 20 min. Furthermore, we presented a new naturally-sourced mixture of Cs+KPF-NCs with powerful antibacterial effects against MDRandat very low concentrations. The membrane evidenced a remarkable antibacterial naturewith almost 100% cell viability reduction against the study strains after 48 h. By virtue of these advantages, this green blend is highly proposed for optimal wound care.
尽管近几十年来医学取得了重大进展，但成功治疗感染性伤口仍然是一个挑战。在这项研究中，开发了一种聚羟基丁酸酯（PHB）和壳聚糖（Cs）的功能混合物，用于减轻伤口感染，具有定制的生物和物理化学特性。水不溶性山奈酚（KPF）预先制成水可溶的 KPF 纳米晶体（KPF-NCs），粒径为 145 ± 11nm，具有高胶体稳定性（-31 ± 0.4 mV），以提高其药物经皮传递。PHB-Cs-KPF-NCs（1:2 比例）薄膜在透气性、热稳定性和机械强度（33 ± 1 MPa）方面具有最佳的物理性能。此外，XRD 和 FTIR 结果表明 Cs、PHB 和 KPF 之间存在相互作用，降低了薄膜的结晶度。薄膜的扫描电子显微镜显示出高度互联的多孔形态。KPF-NCs 被整合到 PHB-Cs 基质中，包封效率高达 96.6%。增强的载药薄膜显示出 KPF-NCs 在 48 小时内的持续释放模式。有趣的是，开发的共混物在 20 分钟内具有令人印象深刻的凝血能力。此外，我们提出了一种新的天然来源的 Cs+KPF-NCs 混合物，具有强大的抗 MDR 抗菌作用，浓度非常低。该膜具有显著的抗菌性质，在 48 小时后对研究菌株的细胞存活率几乎降低到 100%。由于这些优势，这种绿色混合物非常适合优化伤口护理。
