Key Laboratories of Fine Chemicals and Surfactants in Sichuan Provincial Universities, School of Chemical Engineering, Sichuan University of Science & Engineering, Zigong, China.
Department of Neurosurgery, Zigong Fourth People's Hospital, Zigong, China.
J Biomater Appl. 2021 Nov;36(5):859-871. doi: 10.1177/08853282211003850. Epub 2021 Apr 14.
Spider silk protein has attracted much attention on account of its excellent mechanical properties, biodegradability, and biocompatibility. As the main protein component of spider silk, spidroin plays important role in spider spinning under natural circumstances and biomaterial application in medicine as well. Compare to the native spidroin which has a large molecular weight (>300 kDa) with highly repeat glycine and polyalanine regions, the recombinant spidroin was maintained the core amino motifs and much easier to collect. Here, we reviewed the application of recombinant spider silk protein eADF4(C16), major ampullate spidroin (MaSp), minor ampullate spidroin (MiSp), and the derivatives of recombinant spider silk protein in drug delivery system. Moreover, we also reviewed the application of spider silk protein in the field of alternative materials, repairing materials, wound dressing, surgical sutures along with advances in recombinant spider silk protein.
蜘蛛丝蛋白因其优异的机械性能、生物可降解性和生物相容性而备受关注。作为蜘蛛丝的主要蛋白质成分，蛛丝蛋白在蜘蛛自然吐丝和生物材料在医学中的应用中起着重要作用。与天然蛛丝蛋白相比，重组蛛丝蛋白分子量较大（>300kDa），富含甘氨酸和丙氨酸重复序列，保留了核心氨基酸基序，且更容易收集。在这里，我们综述了重组蜘蛛丝蛋白 eADF4（C16）、大囊蛛丝蛋白（MaSp）、小囊蛛丝蛋白（MiSp）及其衍生物在药物传递系统中的应用。此外，我们还综述了蜘蛛丝蛋白在替代材料、修复材料、伤口敷料、手术缝线等领域的应用，以及重组蜘蛛丝蛋白的进展。
