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重组蛛丝蛋白微凝胶作为细胞工程构建体的基础介导大鼠肝脏再生

Recombinant Spidroin Microgel as the Base of Cell-Engineered Constructs Mediates Liver Regeneration in Rats.

作者信息

Shagidulin Murat, Onishchenko Nina, Grechina Anastasiia, Nikolskaya Alla, Krasheninnikov Mikhail, Lyundup Aleksey, Volkova Elena, Mogeiko Natalia, Venediktov Artem, Piavchenko Gennadii, Davydova Lubov, Ramonova Alla, Bogush Vladimir, Gautier Sergey

机构信息

Federal State Budgetary Institution "Shumakov National Medical Research Center of Transplantology and Artificial Organs" of the Ministry of Health of the Russian Federation, 123182 Moscow, Russia.

Federal State Autonomous Educational Institution of Higher Education, "I.M. Sechenov First Moscow State Medical University" of the Ministry of Health of the Russian Federation (Sechenov University), 119435 Moscow, Russia.

出版信息

Polymers (Basel). 2022 Aug 4;14(15):3179. doi: 10.3390/polym14153179.

DOI:10.3390/polym14153179
PMID:35956695
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9370922/
Abstract

: In this study, we seek to check if recombinant spidroin rS1/9 is applicable for cell-engineering construct development. Novel technologies of cell and tissue engineering are relevant for chronic liver failure management. Liver regeneration may represent one of the possible treatment options if a cell-engineered construct (CEC) is used. Nowadays, one can see the continuous study of various matrices to create an appropriate CEC. : We have adhered allogenic liver cells and multipotent mesenchymal bone marrow stem cells (MMSC BM) to a microgel with recombinant spidroin rS1/9. Then we have studied the developed implantable CEC in a rat model ( = 80) of chronic liver failure achieved by prolonged poisoning with carbon tetrachloride. Our results demonstrate that the CECs change the values of biochemical tests and morphological parameters in chronic liver failure in rats. We consider there to be a positive effect from the microgel-based CECs with recombinant spidroin rS1/9 in the treatment of chronic liver failure.

摘要

在本研究中，我们试图检验重组蛛丝蛋白rS1/9是否适用于细胞工程构建体的开发。细胞和组织工程的新技术与慢性肝衰竭的管理相关。如果使用细胞工程构建体（CEC），肝脏再生可能是一种可行的治疗选择。如今，可以看到人们不断研究各种基质以创建合适的CEC。我们已将同种异体肝细胞和多能间充质骨髓干细胞（MMSC BM）附着于含有重组蛛丝蛋白rS1/9的微凝胶上。然后，我们在通过四氯化碳长期中毒建立的大鼠慢性肝衰竭模型（n = 80）中研究了所开发的可植入CEC。我们的结果表明，CEC改变了大鼠慢性肝衰竭中生化检测值和形态学参数。我们认为含重组蛛丝蛋白rS1/9的基于微凝胶的CEC在治疗慢性肝衰竭方面具有积极作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bfce/9370922/fe5cfcaabd16/polymers-14-03179-g005a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bfce/9370922/5343004f0c62/polymers-14-03179-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bfce/9370922/14c05e16fdb4/polymers-14-03179-g002a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bfce/9370922/05bbea094afe/polymers-14-03179-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bfce/9370922/5c26f9f6680c/polymers-14-03179-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bfce/9370922/fe5cfcaabd16/polymers-14-03179-g005a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bfce/9370922/5343004f0c62/polymers-14-03179-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bfce/9370922/14c05e16fdb4/polymers-14-03179-g002a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bfce/9370922/05bbea094afe/polymers-14-03179-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bfce/9370922/5c26f9f6680c/polymers-14-03179-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bfce/9370922/fe5cfcaabd16/polymers-14-03179-g005a.jpg

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