Genome-wide identification and expression pattern of SnRK gene family under several hormone treatments and its role in floral scent emission in .

The (Snf1-Related protein Kinase) gene family plays crucial roles in various plant signaling pathways and stress-adaptive responses including biotic and abiotic stresses via activating protein phosphorylation pathways. However, there is no information available on the role of the gene family in . is an important crop widely cultivated as an ornamental plant, herb, spice, or condiment. In this study, 60 genes were identified from the genomic and transcriptome data. Phylogenetic and gene structure analysis showed that the genes were divided into three groups (, and ) and among them subfamily was further subdivided into two clades according to the number of introns. Chromosome localization analysis showed that genes were unevenly mapped onto all chromosomes, and the ratio of 24 paralogues includes four tandems and 20 segmental duplications indicated that the gene family underwent a purifying selection. -regulatory elements analysis suggested that the genes respond to multiple hormones and other stresses. The responsiveness of genes to several hormones was analyzed by quantitative real-time PCR. Based on the different transcriptome data, two candidates genes ( and ) were screened out for further characterization . The subcellular localization experiment revealed that both genes were located in the nucleus and cytoplasm. Moreover, virus-induced gene silencing (VIGS) of and significantly reduced the floral volatile contents by suppressing the expression of terpene synthase genes ( and ), indicating that and genes play an important role in the regulatory mechanism of floral aroma. These results will provide novel insights into the functional dissection of gene family.