Rodríguez-López José Miguel, Lachica Manuel, González-Valero Lucrecia, Fernández-Fígares Ignacio
Départment Sciences Agronomiques et Animales, Institut Polytechnique LaSalle Beauvais, Beauvais, France.
Department of Physiology and Biochemistry of Animal Nutrition, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, Granada, Spain.
PeerJ. 2021 Mar 10;9:e11014. doi: 10.7717/peerj.11014. eCollection 2021.
As insulin sensitivity may help to explain divergences in growth and body composition between native and modern breeds, metabolic responses to glucose infusion were measured using an intra-arterial glucose tolerance test (IAGTT). Iberian ( = 4) and Landrace ( = 5) barrows (47.0 ± 1.2 kg body weight (BW)), fitted with a permanent carotid artery catheter were injected with glucose (500 mg/kg BW) and blood samples collected at -10, 0, 5, 10, 15, 20, 25, 30, 45, 60, 90, 120 and 180 min following glucose infusion. Plasma samples were analysed for insulin, glucose, lactate, triglycerides, cholesterol, creatinine, albumin and urea. Insulin sensitivity indices were calculated and analysed. Mean plasma glucose, creatinine and cholesterol concentrations were lower ( < 0.01) in Iberian (14, 68 and 22%, respectively) than in Landrace pigs during the IAGTT. However, mean plasma insulin, lactate, triglycerides and urea concentrations were greater ( < 0.001) in Iberian (50, 35, 18 and 23%, respectively) than in Landrace pigs. Iberian pigs had larger area under the curve (AUC) of insulin ( < 0.05) or tended to a greater AUC of lactate ( < 0.10), and a smaller ( < 0.05) AUC for glucose 0-60 min compared with Landrace pigs. Indices for estimating insulin sensitivity in fasting conditions indicated improved β-cell function in Iberian compared with Landrace pigs, but no difference ( > 0.10) in calculated insulin sensitivity index was found after IAGTT between breeds. A time response ( < 0.05) was obtained for insulin, glucose and lactate so that maximum concentration was achieved at 10 and 15 min post-infusion for insulin (Iberian and Landrace pigs, respectively), immediately post-infusion for glucose, and 20 min post-infusion for lactate, decreasing thereafter until basal levels. There was no time effect for the rest of metabolites evaluated. In conclusion, growing Iberian pigs challenged with an IAGTT showed changes in biochemical parameters and insulin response that may indicate an early stage of insulin resistance.
由于胰岛素敏感性可能有助于解释本地品种和现代品种在生长及身体组成方面的差异，因此通过动脉内葡萄糖耐量试验（IAGTT）来测定对葡萄糖输注的代谢反应。给4头伊比利亚猪和5头长白猪（体重47.0±1.2千克）安装永久性颈动脉导管后，静脉注射葡萄糖（500毫克/千克体重），并在输注葡萄糖后的-10、0、5、10、15、20、25、30、45、60、90、120和180分钟采集血样。分析血浆样本中的胰岛素、葡萄糖、乳酸、甘油三酯、胆固醇、肌酐、白蛋白和尿素含量。计算并分析胰岛素敏感性指数。在IAGTT期间，伊比利亚猪的平均血浆葡萄糖、肌酐和胆固醇浓度低于长白猪（P<0.01），分别低14%、68%和22%。然而，伊比利亚猪的平均血浆胰岛素、乳酸、甘油三酯和尿素浓度高于长白猪（P<0.001），分别高50%、35%、18%和23%。与长白猪相比，伊比利亚猪的胰岛素曲线下面积（AUC）更大（P<0.05），或乳酸AUC有增大趋势（P<0.10），而0至60分钟葡萄糖的AUC更小（P<0.05）。空腹条件下评估胰岛素敏感性的指标表明，与长白猪相比，伊比利亚猪的β细胞功能有所改善，但IAGTT后各品种间计算出的胰岛素敏感性指数无差异（P>0.10）。胰岛素、葡萄糖和乳酸出现了时间反应（P<0.05），胰岛素在输注后10分钟（伊比利亚猪）和15分钟（长白猪）达到最高浓度，葡萄糖在输注后立即达到最高浓度，乳酸在输注后20分钟达到最高浓度，此后逐渐下降至基础水平。其余评估的代谢物没有时间效应。总之，接受IAGTT刺激的生长中的伊比利亚猪在生化参数和胰岛素反应方面出现了变化，这可能表明处于胰岛素抵抗的早期阶段。
