中国新疆三种植物根部内生真菌群落及有效成分的差异

Differences in the endophytic fungal community and effective ingredients in root of three species in Xinjiang, China.

作者信息

Dang Hanli, Zhang Tao, Wang Zhongke, Li Guifang, Zhao Wenqin, Lv Xinhua, Zhuang Li

机构信息

College of Life Sciences, Shihezi University, Shihezi, Xinjiang, China.

出版信息

PeerJ. 2021 Mar 9;9:e11047. doi: 10.7717/peerj.11047. eCollection 2021.

DOI:10.7717/peerj.11047

PMID:33854843

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7953873/

Abstract

BACKGROUND

Endophytic fungi influence the quality and quantity of the medicinal plant's bioactive compounds through specific fungus-host interactions. Nevertheless, due to the paucity of information, the composition of endophytic fungal communities and the mechanism by which effective ingredients regulate endophytic fungal communities in roots remains unclear.

METHODS

In this study, we collected root and soil samples (depth range: 0-20, 20-40, and 40-60 cm) of three species (, and ). Glycyrrhizic acid and liquiritin content were determined using high-performance liquid chromatography (HPLC), and total flavonoid content was determined using ultraviolet spectrophotometry. High-throughput sequencing technology was employed to explore the composition and diversity of the endophytic fungal community in different root segments of three species. Furthermore, soil samples were subjected to physicochemical analyses.

RESULTS

We observed that the liquiritin content was not affected by the root depth (0-20 cm, 20-40 cm, and 40-60 cm). Still, it was significantly affected by the species () ( < 0.05). In root, a total of eight phyla and 140 genera were annotated so far, out of which Ascomycota and Basidiomycota phyla, and the , and genera were found to be significantly dominant. Spearman correlation analysis revealed that liquiritin content was accountable for the differences in the diversity of the endophytic fungal community. Furthermore, distance-based redundancy analysis (db-RDA) showed that physicochemical properties of the soil (available potassium and ammonium nitrogen) and the root factors (liquiritin and water content) were the main contributing factors for the variations in the overall structure of the endophytic fungal community. Our results showed that the effective ingredients of root and physicochemical properties of the soil regulated the endophytic fungal community composition and medicinal licorice diversity.

摘要

背景

内生真菌通过特定的真菌-宿主相互作用影响药用植物生物活性化合物的质量和数量。然而，由于信息匮乏，根部内生真菌群落的组成以及有效成分调节根部内生真菌群落的机制仍不清楚。

方法

在本研究中，我们采集了三种植物（、和）的根和土壤样本（深度范围：0-20、20-40和40-60厘米）。使用高效液相色谱法（HPLC）测定甘草酸和甘草苷含量，使用紫外分光光度法测定总黄酮含量。采用高通量测序技术探究三种植物不同根段内生真菌群落的组成和多样性。此外，对土壤样本进行理化分析。

结果

我们观察到甘草苷含量不受根深度（0-20厘米、20-40厘米和40-60厘米）的影响，但受植物种类（）的显著影响（<0.05）。在根中，目前共注释出8个门和140个属，其中子囊菌门和担子菌门以及属、属和属显著占优势。Spearman相关性分析表明，甘草苷含量是内生真菌群落多样性差异的原因。此外，基于距离的冗余分析（db-RDA）表明，土壤的理化性质（有效钾和铵态氮）和根因素（甘草苷和含水量）是内生真菌群落整体结构变化的主要贡献因素。我们的结果表明，根的有效成分和土壤的理化性质调节了内生真菌群落组成和药用甘草的多样性。