Semenescu Iulia, Avram Stefana, Similie Diana, Minda Daliana, Diaconeasa Zorita, Muntean Delia, Lazar Antonina Evelina, Gurgus Daniela, Danciu Corina
Department of Pharmacognosy, "Victor Babes" University of Medicine and Pharmacy, 2nd Eftimie Murgu Square, 300041 Timisoara, Romania.
Research and Processing Center for Medicinal and Aromatic Plants, "Victor Babes" University of Medicine and Pharmacy, 2nd Eftimie Murgu Square, 300041 Timisoara, Romania.
Plants (Basel). 2024 Nov 21;13(23):3265. doi: 10.3390/plants13233265.
L., also known as licorice, belongs to the family and is one of the most commercially valuable plants worldwide, being used in the pharmaceutical, cosmetic, and food industries, both for its therapeutic benefits as well as for the sweetening properties of the extract. This study evaluates the phytochemical composition, the biological activities, and the safety profile of a methanolic extract of licorice root (LRE) obtained from Romania. Ten phytocompounds were quantified by the HPLC-DAD-ESI+, the most abundant being the triterpene glycyrrhizin (13.927 mg/g dry extract.), followed by these flavonoids: liquiritin, liquiritigenin-apiosyl-glucoside, and apigenin-rutinoside liquiritigenin. The total phenolic content of the LRE was found to be 169.83 mg gallic acid/g dry extract. (GAE/g d.e.), and the extract showed a maximum of 79.29% antioxidant activity in the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. Good antimicrobial activity of the LRE was observed for Gram-negative bacteria, especially for and . The mineral content of the LRE was indicative of the lack of toxicity; heavy metals such as lead, cadmium, arsenic, nickel, and cobalt were below the detection limit. The safety profile of the licorice extract was assessed using the in vivo hen egg test-chorioallantoic membrane (HET-CAM protocol), indicating no irritability, good tolerability, and biocompatibility. The phytochemical and biological characterization of the Romanian licorice root extract reveals a good source of glycyrrhizin and polyphenols with antioxidant and antimicrobial potential, along with a safety profile that may be useful for future therapeutic applications.
甘草，也被称为甘草，属于豆科，是全球最具商业价值的植物之一，因其治疗功效以及提取物的甜味特性而被用于制药、化妆品和食品工业。本研究评估了从罗马尼亚获得的甘草根甲醇提取物（LRE）的植物化学成分、生物活性和安全性。通过HPLC-DAD-ESI+对十种植物化合物进行了定量分析，含量最高的是三萜类甘草酸（13.927毫克/克干提取物），其次是这些黄酮类化合物：甘草苷、甘草素-芹菜糖基-葡萄糖苷和芹菜素-芸香糖苷甘草素。LRE的总酚含量为169.83毫克没食子酸/克干提取物（GAE/克干提取物），在2,2-二苯基-1-苦基肼（DPPH）测定中，提取物的抗氧化活性最高为79.29%。观察到LRE对革兰氏阴性菌具有良好的抗菌活性，尤其是对[具体细菌名称1]和[具体细菌名称2]。LRE的矿物质含量表明其无毒；铅、镉、砷、镍和钴等重金属低于检测限。使用体内鸡胚试验-绒毛尿囊膜（HET-CAM方案）评估了甘草提取物的安全性，结果表明无刺激性、耐受性良好且具有生物相容性。罗马尼亚甘草根提取物的植物化学和生物学特性表明，它是甘草酸和多酚的良好来源，具有抗氧化和抗菌潜力，其安全性特征可能对未来的治疗应用有用。
