Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, College of Tropical Crops, Hainan University, Haikou, 570228, China.
Biotechnol Lett. 2021 Jul;43(7):1503-1512. doi: 10.1007/s10529-021-03128-8. Epub 2021 Apr 15.
Botrytis cinerea cause postharvest diseases on fruit and lead economic losses. Application of environment-friendly natural compounds is an alternative for synthetic fungicides to control postharvest disease. Lycorine is an indolizidine alkaloid which is widely used for human drug design, however, application of lycorine in controlling postharvest disease and the underlying mechanisms have not been reported. In this study, the effects of lycorine on mycelium growth, spore germination, disease development in apple fruit, cell viability, cell membrane integrity, cell wall deposition, and expression of mitogen-activated protein kinase (MAPK) and GTPase of B. cinerea were investigated. Our results showed that lycorine was effective in controlling postharvest gray mold caused by B. cinerea on apple fruit. In the in vitro tests, lycorine strongly inhibited spore germination and mycelium spreading in culture medium. Investigation via fluorescein diacetate and propidium iodide staining suggested that lycorine could damage the membrane integrity and impair cell viability of B. cinerea. Furthermore, the expression levels of several MAPK and GTPase coding genes were reduced upon the lycorine treatment. Taken together, lycorine is an effective and promising way to control postharvest disease caused by B. cinerea.
灰葡萄孢引起水果采后病害，导致经济损失。应用环保型天然化合物是替代合成杀菌剂防治采后病害的一种方法。石蒜碱是一种吲哚里西啶生物碱，广泛应用于人类药物设计，然而，石蒜碱在控制采后病害中的应用及其潜在机制尚未报道。在本研究中，研究了石蒜碱对苹果果实中灰霉菌菌丝生长、孢子萌发、病害发展、细胞活力、细胞膜完整性、细胞壁沉积以及丝裂原活化蛋白激酶（MAPK）和 GTPase 表达的影响。结果表明，石蒜碱能有效控制苹果果实采后灰霉病。在体外试验中，石蒜碱强烈抑制孢子萌发和菌丝在培养基中的扩散。通过荧光素二乙酸酯和碘化丙啶染色的研究表明，石蒜碱可以破坏细胞膜的完整性并损害灰霉菌的细胞活力。此外，石蒜碱处理后几种 MAPK 和 GTPase 编码基因的表达水平降低。综上所述，石蒜碱是一种有效且有前途的控制灰葡萄孢引起的采后病害的方法。
