Leunk R D, Johnson P T, David B C, Kraft W G, Morgan D R
Anti-Infective Research Department, Norwich Eaton Pharmaceuticals Inc., New York 13815.
J Med Microbiol. 1988 Jun;26(2):93-9. doi: 10.1099/00222615-26-2-93.
Broth-culture filtrates of Campylobacter pylori induced non-lethal cytopathic effects in vitro in 7 of 9 mammalian cell lines tested. Transmission electronmicroscopy revealed that the response consisted of intracellular vacuolisation. Intestine 407 cells were among the most responsive and were used for routine assay. About 55% of isolates of C. pylori tested, originating from four geographic regions worldwide, produced cytotoxic activity. The activity was neutralisable by specific antisera to broth-culture filtrates or to sonicated bacteria but not by antisera to other bacterial preparations. Cytotoxic activity was heat-labile (70 degrees C for 30 min), was protease-sensitive and ammonium-sulphate precipitable. It did not pass through an ultrafiltration membrane with a nominal mol.-wt limit of 100 X 10(3). It was concluded that C. pylori can produce a factor that alters cultured cells in vitro. The relevance of this factor to the pathogenesis of gastritis associated with C. pylori remains to be determined.
幽门螺杆菌的肉汤培养滤液在体外对所测试的9种哺乳动物细胞系中的7种诱导了非致死性细胞病变效应。透射电子显微镜显示，反应包括细胞内空泡化。肠407细胞是反应最强烈的细胞系之一，并用于常规检测。来自全球四个地理区域的所测试的幽门螺杆菌分离株中，约55%产生了细胞毒性活性。该活性可被针对肉汤培养滤液或超声处理细菌的特异性抗血清中和，但不能被针对其他细菌制剂的抗血清中和。细胞毒性活性对热不稳定（70℃，30分钟），对蛋白酶敏感且可被硫酸铵沉淀。它不能通过标称分子量极限为100×10³的超滤膜。得出的结论是，幽门螺杆菌可产生一种在体外改变培养细胞的因子。该因子与幽门螺杆菌相关胃炎发病机制的相关性仍有待确定。
