Department of Materials Science and Engineering, Biomaterials Lab, African University of Science and Technology, Km 10 Airport Road, Galadimawa, Abuja, Federal Capital Territory, Nigeria.
Department of Mechanical and Aerospace Engineering, Princeton University, 41 Olden Street, Princeton, NJ 08544, USA; Division of Vascular & Interventional Radiology, Mayo Clinic, 13400 East Shea Blvd. Scottsdale, Arizona 85259, USA.
J Mech Behav Biomed Mater. 2021 Jul;119:104505. doi: 10.1016/j.jmbbm.2021.104505. Epub 2021 Apr 9.
This paper presents the results of a study of the actin cytoskeletal structures and the statistical variations in the actin fluorescence intensities and viscoelastic properties of non-tumorigenic breast cells and triple-negative breast cancer cells at different stages of tumor progression. The variation in the actin content of the cell cytoskeletal structures is shown to be consistent with the viscoelastic properties of the cell as it progresses from non-tumorigenic to more metastatic states. The corresponding viscoelastic properties of the nuclei and the cytoplasm (Young's moduli, viscosities, and relaxation times) of the cells are also measured using Digital Image Correlation (DIC) and shear assay techniques. These properties are shown to exhibit statistical variations that are well characterized by normal distributions. The changes in the mean properties of individual cancer cells are tested using Fisher pairwise comparisons and the analysis of variance (ANOVA). The implications of the results are then discussed for the development of shear assay techniques and mechanical biomarkers for the detection of triple-negative breast cancer at different stages of tumor progression.
本文介绍了一项研究的结果，该研究考察了非致瘤性乳腺细胞和三阴性乳腺癌细胞在肿瘤进展的不同阶段的肌动蛋白细胞骨架结构和肌动蛋白荧光强度及粘弹性特性的统计变化。细胞骨架结构中肌动蛋白含量的变化与细胞从非致瘤性向更具转移性状态进展时的粘弹性特性一致。还使用数字图像相关（DIC）和剪切试验技术测量了细胞核和细胞质（杨氏模量、粘度和弛豫时间）的相应粘弹性特性。结果表明，这些特性表现出良好的正态分布特征的统计变化。使用 Fisher 两两比较和方差分析（ANOVA）测试了单个癌细胞的平均特性变化。然后讨论了这些结果对剪切试验技术和机械生物标志物的发展的意义，以用于检测肿瘤进展不同阶段的三阴性乳腺癌。
